Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Drosophila Sumo enzyme cycle components expressed in a bacterial system with bait and prey.

A two step coIP of Sumo was utilized (His followed by Flag).

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.