FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Sumo, Ras85D
FlyBase ID
FBig0000121726
Interaction Network
Interactions Browser links
Sumo network
Ras85D network
Reported Interactions
FBrf0208144-17.coIP.MS
Reference
Nie et al., 2009
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sumo 

SUMO

bait

Flag tag

Ras85D 

Ras1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Nie et al., 2009)
FBrf0208144-18.PD.WB
Reference
Nie et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sumo 

SUMO

bait

HIS tag

Ras85D 

Ras1

prey

GST tag

Ras85D 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ras85D 
C terminal domain
sufficient binding region
aa 165-189

coordinates relative to Ras85D-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Drosophila Sumo enzyme cycle components expressed in a bacterial system with bait and prey.

(Nie et al., 2009)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Nie et al., 2009)
External Crossreferences and Linkouts ( 0 )
References (1)