FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Miga, Uvrag
FlyBase ID
FBig0000121823
Interaction Network
Interactions Browser links
Miga network
Uvrag network
Reported Interactions
FBrf0254939-17.coIP.WB
Reference
Xu et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Miga 

Miga

bait

Flag-HA tag

Uvrag 

Uvrag

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Miga could pull down both Atg14 and Uvrag when all three proteins were co-expressed.

(Xu et al., 2022)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xu et al., 2022)
FBrf0254939-3.coIP.WB
Reference
Xu et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Miga 

Miga

bait

V5 tag

Miga 
Uvrag 

Uvrag

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Miga 
N-terminal domain
sufficient binding region
aa 1-255

coordinates relative to Miga-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xu et al., 2022)

Miga could pull down Uvrag under fed and starvation conditions.

(Xu et al., 2022)
FBrf0254939-4.coIP.WB
Reference
Xu et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Uvrag 

Uvrag

bait

V5 tag

Miga 

Miga

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Miga plasmid is a genomic rescue construct that mimisc endogenous expression.

(Xu et al., 2022)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xu et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (1)