FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
sau, 14-3-3ζ
FlyBase ID
FBig0000121825
Interaction Network
Interactions Browser links
sau network
14-3-3ζ network
Reported Interactions
FBrf0255112-3.PD.WB
Reference
Frappaolo et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
14-3-3ζ 

14-3-3ζ

bait

GST tag

sau 

dGOLPH3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

(Frappaolo et al., 2022)
FBrf0255112-4.PD.WB
Reference
Frappaolo et al., 2022
Description
physical association
Assay
Collection
Source/Stage
testis / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
14-3-3ζ 

14-3-3ζ

bait

GST tag

sau 

dGOLPH3

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild type testes extract.

(Frappaolo et al., 2022)
FBrf0255112-9.Y2H
Reference
Frappaolo et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sau 

dGOLPH3

bait

fused to LexA DNA-binding domain

14-3-3ζ 

14-3-3ζ

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Frappaolo et al., 2022)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Frappaolo et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (1)