FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Mi-2, Hsp70Aa
FlyBase ID
FBig0000121885
Interaction Network
Interactions Browser links
Mi-2 network
Hsp70Aa network
Reported Interactions
FBrf0253677-19.ICLIP.SEQ
Reference
Ullah et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Mi-2 

dMi-2

bait

GFP tag

Hsp70Aa 

Hsp70Aa

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenously tagged construct; prey produced from endogenous gene.

(Ullah et al., 2022)
FBrf0253677-21.EMSA
Reference
Ullah et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mi-2 

dMi-2

bait

Flag tag

Hsp70Aa 

Hsp70Aa

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Complexes of Mi-2 and Hsp70Aa were identified by Sybr gold stain; 5 100bp fragments, as well as double stranded Hsp70Aa were tested and all bound equally well to Mi-2.

(Ullah et al., 2022)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey composed of synthesized oligonucleotides.

(Ullah et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (1)