Two-hybrid system: yeast GAL4-BD/GAL4-AD; Khc aa 601-849 interacts with Plp aa 2539-2895; Khc aa 850-975 interacts with Plp aa 584-1376 and aa 1377-1811.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Source was live S2 cells; bait produced from transfected construct; prey produced from transfected construct.

Bait protein was fused to mitochondrial membrane anchor Tom20-RFP, which targets the chimera to mitochondria. The ability of this chimera to recruit prey protein to mitochondria was taken as evidence of direct physical interaction. Khc aa 850-975 interacts with Plp aa 584-1376 and aa 1377-1811.

Source was live S2 cells; bait produced from transfected construct; prey produced from transfected construct.

Bait protein was fused to mitochondrial membrane anchor Tom20-RFP, which targets the chimera to mitochondria. The ability of this chimera to recruit prey protein to mitochondria was taken as evidence of direct physical interaction. Khc aa 601-849 interacts with Plp aa 2539-2895.

Interaction with Plp strongest with Khc delta H2 mutant and addition of Klc to reaction; in the Khc delta H2 mutant, the tail is unable to interact with the motor.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.