FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
msk, Act5C
FlyBase ID
FBig0000121919
Interaction Network
Interactions Browser links
msk network
Act5C network
Reported Interactions
FBrf0254421-14.BiFC.FM
Reference
Borkúti et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
msk 

Moleskin

bait

YFP tag, N-terminal fragment

Act5C 

Act5C

prey

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2R+ cells; proteins produced from transfected constructs.

(Borkúti et al., 2022)
FBrf0254421-2.PD.A
Reference
Borkúti et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Act5C 

Act5C

bait

GST tag

Act5C 
msk 

Moleskin

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Act5C 
monomeric form; residue 63
sufficient binding region
aa 63

R63D; coordinates relative to AA-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Borkúti et al., 2022)
FBrf0254421-9.BiFC.FM
Reference
Borkúti et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Act5C 

Act5C

bait

YFP tag, N-terminal fragment

msk 

Moleskin

prey

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2R+ cells; proteins produced from transfected constructs.

(Borkúti et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (1)