FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
wash, pav
FlyBase ID
FBig0000122061
Interaction Network
Interactions Browser links
wash network
pav network
Reported Interactions
FBrf0256485-1.coIP.MS
Reference
Davidson et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
wash 

Wash

bait

Tap tag

pav 

Pav

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Davidson et al., 2023)
FBrf0256485-11.PD.WB
Reference
Davidson et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pav 

Pav

bait

GST tag

wash 

Wash

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.

(Davidson et al., 2023)
FBrf0256485-12.PD.A
Reference
Davidson et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
wash 

Wash

bait

GST tag

wash 
wash 
pav 

Pav

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
wash 
WHD1 and 2 domains
sufficient binding region
unspecified

coordinates relative to wash-PA

wash 
pav and tum interaction domain residues 123-127
mutation decreasing interaction
aa 123-127

YSSHS>AAAA, coordinates relative to wash-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Davidson et al., 2023)
FBrf0256485-5.CM.WB
Reference
Davidson et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
wash 

Wash

neutral component
wash 

Wash

neutral component
wash 

Wash

neutral component
pav 

Pav

neutral component
pav 

Pav

neutral component
pav 

Pav

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

wash, FAM21 and tum comigrate in a 900kD complex. wash, pav and tum comigrate in a 750kD complex.

(Davidson et al., 2023)

Source was nuclear cell extract of Kc167 cell line; proteins produced from endogenous genes.

(Davidson et al., 2023)
FBrf0256485-7.coIP.WB
Reference
Davidson et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
wash 

Wash

bait
pav 

Pav

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear cell extract of Kc167 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Davidson et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (1)