FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
hpo, EMC1
FlyBase ID
FBig0000123051
Interaction Network
Interactions Browser links
hpo network
EMC1 network
Reported Interactions
FBrf0258337-1.coIP.WB
Reference
Shi et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
EMC1 

EMC1

bait

FLAG tag

hpo 

Hpo

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Shi et al., 2023)
FBrf0258337-2.coIP.WB
Reference
Shi et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
hpo 

Hpo

bait

FLAG tag

EMC1 

EMC1

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Shi et al., 2023)
FBrf0258337-3.coIP.WB
Reference
Shi et al., 2023
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
EMC1 

EMC1

bait

FLAG tag

hpo 

Hpo

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait and prey produced from tagged transgenic constructs.

(Shi et al., 2023)
FBrf0258337-4.coIP.WB
Reference
Shi et al., 2023
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hpo 

Hpo

bait

FLAG tag

EMC1 

EMC1

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait and prey produced from tagged transgenic constructs.

(Shi et al., 2023)
FBrf0258337-5.coIP.WB
Reference
Shi et al., 2023
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hpo 

Hpo

bait

GFP-FLAG tag

EMC1 

EMC1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait produced from endogenously tagged transgenic construct; prey produced from endogenous gene.

(Shi et al., 2023)
FBrf0258337-7.coIP.WB
Reference
Shi et al., 2023
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hpo 

Hpo

bait

FLAG tag

EMC1 

EMC1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Phosporylation dead and phosphorylation mimic mutants of hpo do not affect its interaction with EMC1.

(Shi et al., 2023)

Source was adults of transgenic fly line; bait produced tagged transgenic construct; prey produced from endogenous gene.

(Shi et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (1)