FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
tap, soul
FlyBase ID
FBig0000123315
Interaction Network
Interactions Browser links
tap network
soul network
Reported Interactions
FBrf0260479-1.coIP.WB
Reference
Liu et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
tap 

Tap

bait

FLAG tag

soul 

Soul

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Liu et al., 2024)
FBrf0260479-2.coIP.WB
Reference
Liu et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
tap 

Tap

bait

Myc tag

soul 

Soul

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Liu et al., 2024)
FBrf0260479-3.coIP.WB
Reference
Liu et al., 2024
Description
physical association
Assay
Collection
Source/Stage
larval ring gland / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
soul 

Soul

bait

HA tag

tap 

Tap

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was the larval ring gland of transgenic fly line; bait and prey produced from tagged transgenic constructs.

(Liu et al., 2024)
FBrf0260479-4.EMSA.UVS
Reference
Liu et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
soul 

Sou

neutral component

Myc tag

soul 

Sou

neutral component

Myc tag

soul 

Sou

neutral component

Myc tag

tap 

Tap

neutral component

Myc tag

tap 

Tap

neutral component

Myc tag

tap 

Tap

neutral component

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

soul and tap were mixed with biotin labeled DNA conjugated to streptavidin-HRP and a chemiluminescent substrate was used for detection. DNA-protein complexes observed by EMSA were used to deduce protein-protein interactions.

(Liu et al., 2024)

soul and tap bind DNA cooperatively; neither binds DNA on its own in this assay.

(Liu et al., 2024)

Interaction in vitro; proteins produced and purified from transfected constructs.

(Liu et al., 2024)
External Crossreferences and Linkouts ( 0 )
References (1)