Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was live S2R+ cells; proteins produced from transfected constructs.
Source was larval salivary glands or ovaries of transgenic fly line; proteins produced from transgenic constructs.
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was nuclear cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.