FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
fzr, Hsp70Aa
FlyBase ID
FBig0000123616
Interaction Network
Interactions Browser links
fzr network
Hsp70Aa network
Reported Interactions
FBrf0261911-10.coIP.WB
Reference
Qian et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
fzr 

fzr

bait
Hsp70Aa 

Hsp70

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Treatment with 2-phenylethynesulfonamide inhibits fzr-Hsp70 interaction.

(Qian et al., 2025)

Source was cell extract of S2 cell line; bait and prey produced from endogenous gene.

(Qian et al., 2025)
FBrf0261911-11.coIP.WB
Reference
Qian et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Hsp70Aa 

Hsp70

bait
fzr 

fzr

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait and prey produced from endogenous gene.

(Qian et al., 2025)
FBrf0261911-12.coIP.WB
Reference
Qian et al., 2025
Description
physical association
Assay
Collection
Source/Stage
salivary gland / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fzr 

fzr

bait
Hsp70Aa 

Hsp70

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval salivary glands of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Qian et al., 2025)

Treatment with 2-phenylethynesulfonamide inhibits fzr-Hsp70 interaction.

(Qian et al., 2025)
FBrf0261911-8.coIP.WB
Reference
Qian et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
fzr 

fzr

bait

FLAG tag

Hsp70Aa 

Hsp70

prey

V5 tag

Hsp70Aa 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Hsp70Aa 
substrate binding domain
necessary binding region
aa 383-549

coordinates relative to Hsp70Aa-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Treatment with 2-phenylethynesulfonamide inhibits fzr-Hsp70 interaction.

(Qian et al., 2025)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Qian et al., 2025)
FBrf0261911-9.coIP.WB
Reference
Qian et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Hsp70Aa 

Hsp70

bait

V5 tag

fzr 

fzr

prey

FLAG tag

fzr 
fzr 
fzr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
fzr 
WD40 domain 4
necessary binding region
aa 287-326

coordinates relative to fzr-PA

fzr 
WD40 domain 6
necessary binding region
aa 374-414

coordinates relative to fzr-PA

fzr 
WD40 domain 7
necessary binding region
aa 417-456

coordinates relative to fzr-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Qian et al., 2025)
External Crossreferences and Linkouts ( 0 )
References (1)