FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Mycbp, otu
FlyBase ID
FBig0000123631
Interaction Network
Interactions Browser links
Mycbp network
otu network
Reported Interactions
FBrf0262100-6.coIP.WB
Reference
Mercer et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
otu 

otu

bait

FLAG tag

Mycbp 

Mycbp

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Mercer et al., 2025)

! IN8a. Public comments :Serial coIP experiments indicate that CG14545, CG17202, and otu proteins associate as a trimeric complex. Captured as three pairwise interactions.

(Mercer et al., 2025)
FBrf0262100-8.coIP.MS
Reference
Mercer et al., 2025
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
otu 

otu

bait

GFP tag

Mycbp 

Mycbp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from endogenously tagged gene; prey produced from endogenous gene.

(Mercer et al., 2025)
FBrf0263985-1.coIP.WB
Reference
Azlan and Fukunaga, 2025
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mycbp 

Mycbp

bait

EGFP tag

otu 

otu

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Azlan and Fukunaga, 2025)
FBrf0263985-10.coIP.WB
Reference
Azlan and Fukunaga, 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Mycbp 

Mycbp

prey

HA tag

otu 

otu

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

bbn, Mycbp and otu form a complex demonstrated by sequential coimmunoprecipitation. Captured in pairwise interactions.

(Azlan and Fukunaga, 2025)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Azlan and Fukunaga, 2025)
FBrf0263985-3.coIP.WB
Reference
Azlan and Fukunaga, 2025
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mycbp 

Mycbp

bait
otu 

otu

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of wild type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Azlan and Fukunaga, 2025)
FBrf0263985-5.coIP.WB
Reference
Azlan and Fukunaga, 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Mycbp 

Mycbp

bait

HA tag

otu 

otu

prey

FLAG tag

otu 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
otu 
N-terminal domain
sufficient binding region
aa 1-405
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Azlan and Fukunaga, 2025)
External Crossreferences and Linkouts ( 0 )
References (2)