FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000123636
Interaction Network
Interactions Browser links
fzr network
Usp8 network
Reported Interactions
FBrf0261911-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fzr 

fzr

bait

FLAG tag

Usp8 

Usp8

prey

HA tag

Usp8 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Usp8 
N terminal dimerization domain
sufficient binding region
aa 3-113

coordinates relative to Usp8-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Co-expression with Hsp70Aa, increases fzr-Usp8 interaction.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0261911-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fzr 

fzr

bait
Usp8 

Usp8

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait and prey produced endogenous gene.

Treatment with 2-phenylethynesulfonamide inhibits fzr-Usp8 interaction.

FBrf0261911-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Usp8 

Usp8

bait
fzr 

fzr

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait and prey produced endogenous gene.

FBrf0261911-4.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fzr 

fzr

bait
Usp8 

Usp8

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval salivary glands of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Treatment with 2-phenylethynesulfonamide inhibits fzr-Usp8 interaction.

FBrf0261911-5.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Usp8 

Usp8

bait
fzr 

fzr

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval salivary glands of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0261911-6.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Usp8 

Usp8

bait

HA tag

Usp8 
fzr 

fzr

prey

FLAG tag

fzr 
fzr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Usp8 
N terminal dimerization domain
sufficient binding region
aa 3-113

coordinates relative to Usp8-PA

fzr 
WD40 domain 2
necessary binding region
aa 203-242

coordinates relative to fzr-PA

fzr 
WD40 domain 5
necessary binding region
aa 329-371

coordinates relative to fzr-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait and prey produced from transfected constructs.

FBrf0261911-7.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fzr 

fzr

bait

GST tag

Usp8 

Usp8

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.

Addition of Hsp70Aa, increases fzr-Usp8 interaction.

External Crossreferences and Linkouts ( 0 )
References (1)