FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
squ, spn-E
FlyBase ID
FBig0000123645
Interaction Network
Interactions Browser links
squ network
spn-E network
Reported Interactions
FBrf0262122-1.coIP.WB
Reference
Kawaguchi et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
squ 

Squ

bait

FLAG tag

squ 
squ 
squ 
squ 
spn-E 

Spn-E

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
squ 
salt bridge residues
mutation disrupting interaction
aa 107, 109, 115, 163

E107A, E109A, R115A, K163A; coordinates relative to squ-PA

squ 
salt bridge residue 109
mutation decreasing interaction
aa 109

E109A; coordinates relative to squ-PA

squ 
salt bridge residue 115
mutation decreasing interaction
aa 115

R115A; coordinates relative to squ-PA

squ 
salt bridge residue 163
mutation decreasing interaction
aa 163

K163A; coordinates relative to squ-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kawaguchi et al., 2025)
FBrf0262122-4.coIP.WB
Reference
Kawaguchi et al., 2025
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
squ 

Squ

bait
spn-E 

Spn-E

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kawaguchi et al., 2025)
External Crossreferences and Linkouts ( 0 )
References (1)