FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000123765
Interaction Network
Interactions Browser links
nanos network
Rcd-1 network
Reported Interactions
FBrf0262483-27.CL.FM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

nanos

bait

mCherry tag, fused to the plasma membrane

Caf40

prey

EGFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

nanos was fused to a plasma membrane anchor. The ability of this chimera to recruit prey protein to the plasma membrane was taken as evidence of direct physical interaction.

Source was live S2R+ cells; bait and prey produced from endogenous genes.

FBrf0262483-34.CL.FM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Nanos

bait

mCherry tag, fused to the plasma membrane

Caf40

prey

EGFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CAF40-binding α-helix
necessary binding region
aa 140-160
interaction domain surface residue 186
mutation disrupting interaction
aa 18

V186E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2R+ cells; bait and prey produced from endogenous genes.

nanos was fused to a plasma membrane anchor. The ability of this chimera to recruit prey protein to the plasma membrane was taken as evidence of direct physical interaction.

External Crossreferences and Linkouts ( 0 )
References (1)