FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mir-33, trio
FlyBase ID
FBig0000123999
Interaction Network
Interactions Browser links
mir-33 network
trio network
Reported Interactions
FBrf0264480-1.miR-mRNA.LUC
Reference
Wang et al., 2026
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mir-33 

mir-33

bait
mir-33 
trio 

trio

prey
trio 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trio 
3-prime UTR
sufficient binding region
mir-33 
mir-33 target site
necessary binding region
AAUGCA

mutagenesis of the mir-33 seed sequence of AUGCA to GACAG within the context of 736-742 nt; or AUGCA to GACAG within the context of 740-747 nt.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Luciferase reporter assay.

(Wang et al., 2026)

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

(Wang et al., 2026)
External Crossreferences and Linkouts ( 0 )
References (1)