A set of X chromosome transgenic insertion stocks derived by TE mobilization using the P-element construct P{Mae-UAS.6.11}. The P{Mae-UAS.6.11} construct carries the yellow marker y+t7.7, and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
Mobilization of the P-element occurred in males carrying y[-] on the X chromosome, P{Mae-UAS.6.11} on a second chromosome CyO balancer chromosome and P{Δ2-3}99B, a stable source of P transposase, on a marked third chromosome. X chromosome insertions were selected and homozygous or balanced stocks created.
Flanking genomic sequences were obtained by sequencing of inverse PCR products, and were mapped by alignment to the genomic sequence. 2228 insertions were localized to unique sites on the X chromosome. A collection of 848 lines with insertions at least 9kb apart was used for further experiments.
