FlyBase Recombinant Construct Report: pP{DM30}

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Recombinant Construct: pP{DM30}

General Information

Symbol

pP{DM30}

FlyBase ID

FBmc0000171

Feature type

engineered_plasmid

Component Allele(s)

Size

Expression Data

Associated insertion(s)

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

Sequence Data

Sequence (FB)

FlyBase-compiled

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

HindIII

(Mismer and Rubin, 1987)

P5\' (inclusive) (stated as revision)

(FlyBase, 1992-)

Right end

HindIII (inclusive)

(Mismer and Rubin, 1987)

P5\' (exclusive) (stated as revision)

(FlyBase, 1992-)

Segments

Num

Orientation

Symbol

1

+

pCar20-mcs(H3,R1)

1

+

Car1_P5'(P5',R1)

2

+

3

+

Car20_P3'(H3,P3')

4

+

Reference

(FlyBase, 1992-, Mismer and Rubin, 1987)

Phenotypic Data

Component Allele

Phenotypic Data

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

(Mismer and Rubin, 1987)

descendant(s)

(Jones and Gelbart, 1993)

(Jones and Gelbart, 1993)

P{βTub85D-Scer\SCEI.nls}

(Bellaiche et al., 1999)

(Golic et al., 1997)

(Basler et al., 1989)

(Basler et al., 1989)

(Basler et al., 1989)

(Basler et al., 1989)

(Basler et al., 1989)

(Calvi and Gelbart, 1994)

(Bergson and McGinnis, 1990)

(Bergson and McGinnis, 1990)

pP{eve-lacZ5.2}

(Harding et al., 1989)

pP{eve-lacZ6.3+1.2}

(Harding et al., 1989)

(Gavis and Lehmann, 1992)

(Calvi et al., 1991)

(Misra and Rio, 1990)

(Freeman, 1994)

(Zwiebel et al., 1995)

(Kuziora and McGinnis, 1989)

(Loukeris et al., 1995)

pP{nos-tub3'UTR}

(Arrizabalaga and Lehmann, 1999)

pP{osk⁺12.75}

(Ephrussi et al., 1991)

(Comer et al., 1992)

pP{Rh1(-35/+67)-CAT}

(Mismer and Rubin, 1987)

pP{Rh1(-46/+67)-CAT}

(Mismer and Rubin, 1987)

pP{Rh1(-86/+67)-CAT}

(Mismer and Rubin, 1987)

pP{Rh1(-105/+67)-CAT}

(Mismer and Rubin, 1987)

pP{Rh1(-120/+67)-CAT}

(Mismer and Rubin, 1987)

pP{Rh1(-162/+67)-CAT}

(Mismer and Rubin, 1987)

pP{Rh1(-166/+67)-CAT}

(Mismer and Rubin, 1987)

pP{Rh1(-181/+67)-CAT}

(Mismer and Rubin, 1987)

pP{Rh1(-215/+67)-CAT}

(Mismer and Rubin, 1987)

pP{Rh1(-252/+67)-CAT}

(Mismer and Rubin, 1987)

pP{Rh1(-448/+67)-CAT}

(Mismer and Rubin, 1987)

pP{Rh1(-501/-219,Δ,-162/+67)-CAT}

(Mismer and Rubin, 1987)

pP{Rh1(-2800/-701,Δ,-448/+67)-CAT}

(Mismer and Rubin, 1987)

pP{Rh2(-70/+32)-CAT}

(Mismer et al., 1988)

pP{Rh2(-107/+32)-CAT}

(Mismer et al., 1988)

pP{Rh2(-183/+32)-CAT}

(Mismer et al., 1988)

pP{Rh2(-208/+32)-CAT}

(Mismer et al., 1988)

pP{Rh2(-4300/+32)-CAT}

(Mismer et al., 1988)

(FlyBase, 1992-)

(Basler et al., 1989)

(Freeman, 1994)

(Fridell and Searles, 1991)

pP{βTub85D-FLP}

(Golic et al., 1997)

Comments

construct_comment: Used in conjunction with pHSS7 (Seifert et al., 1986, PNAS 83: 735-739) or other cloning vector with polylinker flanked by NotI sites.

(Mismer and Rubin, 1987)

construct_comment: One of a number of cloning vectors based upon pP{Car20}, with different cloning sites.

(Mismer and Rubin, 1987)

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

pDM30

(Mismer and Rubin, 1987)

pP{DM30}

(FlyBase, 1996-)

Secondary FlyBase IDs

References (22)

Report Sections