FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: pBS-KS-attB1-2-GT-SA-GAL4-Hsp70pA
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General Information
Symbol
pBS-KS-attB1-2-GT-SA-GAL4-Hsp70pA
FlyBase ID
FBmc0003059
Feature type
engineered_plasmid
Component Allele(s)
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Description

The pBS-KS-attB1-2-GT-SA-GAL4-Hsp70pA plasmid contains a recombination-mediated cassette exchange (RMCE) cassette that consists of two inverted attB sites which surround a mutagenic gene trap cassette composed of a splice acceptor site, the GAL4 coding sequence and a Hsp70 polyadenylation signal. In the presence of phiC31:int integrase, the attB cassette can undergo RMCE with a target in the genome that contains inverted attP sites, such as the Mi{MIC} element, replacing the target attP cassette with the donor attB cassette sequence.

(Venken et al., 2011)
Construct components
Component
Use(s)
Element type
Tagged with
GAL4
Also carries
attB
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Mi{GT-GAL4}
(Venken et al., 2011)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
pBS-KS-attB1-2-GT-SA-GAL4-Hsp70pA
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (3)