FB2026_02 , released June 18, 2026
Recombinant Construct: pG-en-64bO
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General Information
Symbol
pG-en-64bO
FlyBase ID
FBmc0004064
Feature type
engineered_plasmid
Component Allele(s)
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Description

Gateway destination vector. Contains a synthetic core promoter followed by sequence encoding the dCas9::VP64b transcriptional activator. A tissue-specific enhancer/promoter region can be introduced into this vector to generate a tissue-specific dCas9::VP64b expression construct which can then be inserted into the genome using phiC31:int integrase.

(Huynh et al., 2018)
Construct components
Component
Use(s)
Encoded product / tool
dCas9::VP64b
Also carries
attB
Marker(s)
w+mC
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
gG-Cas9 en_64bO
(Huynh et al., 2018)
pG-en-64bO
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)