FB2026_03 , released September 17, 2026
Recombinant Construct: pHD-Stinger-attP
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General Information
Symbol
pHD-Stinger-attP
FlyBase ID
FBmc0004087
Feature type
engineered_plasmid
Component Allele(s)
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Description

The pHD-Stinger-attP vector is designed for generating dsDNA donor plasmids for CRISPR/Cas9-mediated homology-directed repair (HDR). It was derived from pHD-DsRed-attP by replacing the marker, and contains a Avic\GFPEGFP.3xP3.Tag:NLS(tra) marker flanked by loxP sites (allowing subsequent removal of this marker from a targeted locus), a single attP site and two multiple cloning sites for insertion of 5' and 3' homology arms of the genome region to be targeted. The presence of the attP site allows for subsequent access to a targeted locus.

(Auer et al., 2020)
Construct components
Component
Use(s)
Also carries
attP
loxP
Marker(s)
Avic\GFPEGFP.3xP3.Tag:NLS(tra)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pHD-DsRed-attP
(Auer et al., 2020)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
pHD-Stinger-attP
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)