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Reference
Citation
Kassis, J.A., Noll, E., VanSickle, E.P., Odenwald, W.F., Perrimon, N. (1992). Altering the insertional specificity of a Drosophila transposable element.  Proc. Natl. Acad. Sci. U.S.A. 89(): 1919--1923.
FlyBase ID
FBrf0057521
Publication Type
Research paper
Abstract

Vectors derived from the Drosophila P element transposon are widely used to make transgenic Drosophila. Insertion of most P-element-derived vectors is nonrandom, but they exhibit a broad specificity of target sites. During experiments to identify cis-acting regulatory elements of the Drosophila segmentation gene engrailed, we identified a fragment of engrailed DNA that, when included within a P-element vector, strikingly alters the specificity of target sites. P-element vectors that contain this fragment of engrailed regulatory DNA insert at a high frequency near genes expressed in stripes.

PubMed ID
PubMed Central ID
PMC48565 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Proc. Natl. Acad. Sci. U.S.A.
    Title
    Proceedings of the National Academy of Sciences of the United States of America
    Publication Year
    1915-
    ISBN/ISSN
    0027-8424
    Data From Reference
    Aberrations (1)
    Alleles (20)
    Balancers (1)
    Genes (6)
    Molecular Constructs (2)
    Insertions (3)
    Experimental Tools (1)
    Transgenic Constructs (20)