Subject: Re: tsh3 Michael tsh3 was induced on an enhancer trap line (P(lac, ry+)A from O'Kane/Gehring) localized at the 5' end of tsh. The tsh3 revertant is a ry- derivative of the original ry+ P insertion. If things are simple the breaks should be in 34Db and the ry gene. Primers could therefore be tried from the 3' P element sequence or whatever (ask Cahir?) is at that end of the insertion from the ry gene. Let me know if you need more help. Steve Stephen Kerridge IBDM, CNRS Case 907 Parc Scientifique de Luminy 13288 Marseille Cedex 9 France
