Subject: mus309 et al. Dear Rachel, In deficiency mapping I made use of the following Df(3R)s: M-Kx1, T-32, T-45, P10, kar.D3, P21, T-10, T-55, T-47, -79, T-61, T-7, E-229, kar.D1, kar.Sz-12, cu and M86D. Here are the details: I used two alleles of mus309, 2 and 3 and monitored female sterility. Both mutant alleles are sterile in combination with the following Df(3R)s: M-Kx1,T-32, T-45, P10, kar.D3, P21, T-10, T-47, E-79 T-7. The mus309 alleles are viable and fertile in combination with E-229, kar.D1, kar.Sz-12, kar.H10, cu and M86D. (T-55 and T-61 were not included.) The ck12 lethal alleles (e77, e177 and e294) are lethal in combination with M-Kx1, T-32, T-45, kar.D3, P21, T-10, E-79 and E-229. The ck12 alleles are viable, female and male fertile in combination with the following Df(3R)s: P10, T-61, kar.Sz-12, kar.H10, cu and M86D. It is interesting that Df(3R)T-47 is lethal in combination with e177, viable, female fertile but male sterile with e77 and e294. T-55 and T-7 were not included. Best wishes, Janos.