Subject: ADRC: 668A
Dear Barbara and Susan,
I am writing in connection with your abstract for the upcoming Washington
DC ADRC:
'Genetic characterization of sticks and stones, a gene involved in myoblast fusion.'
You describe the new gene, sns, and give 2-58 as its map location. Could
you tell us which flanking mutations you used in determining this map
location, or (or as well as) the cytological location of sns if you have
determined it by chromosome in situ or by mapping against aberrations.
This is all so that sns can be placed on the genome map. It is nice if we
can keep as many gene records anchored to the map as possible.
Thank you for your help,
with best wishes,
Rachel.
Subject: Re: ADRC: 668A
Rachel,
1. sns is uncovered by Df(2R)Np3, which is a stock in the collection.
2. sns is not uncovered by 3646 - In(2LR)P14LTE45FR. This is also a
stock in the collection that has been mapped cytologically. It overlaps
Df(2R)Np3 genetically, and establishes the proximal breakpoint of the
region that uncovers sns.
3. sns is not uncovered by Df(2R)Np1. This is a third stock in the
collection that has been mapped cytologically. It also overlaps Df(2R)Np3
genetically, and establishes the distal breakpoint of the region that
uncovers sns.
Additionally, before we generated or obtained deficiencies that uncovered
sns, we mapped it by recombination frequency to genetic position 58 using
both Bl and cn on the proximal side and L and sca on the distal side.
Finally, you asked about our reference to the ryanodine receptor. Yes this
the same thing as Rya-r44F (I think we used the terminology in the
published papers). It is also missing in Df(2R)Np3 but present in 3646 and
Df(2R)Np1.
Susan
Susan M. Abmayr, Ph.D.
Asst. Prof. of Molecular Genetics
Dept. of Biochemistry and Molecular Biology
The Pennsylvania State University
459 North Frear Lab
University Park PA.16802