Subject: Re. FlyBase query Dear Gillian, The tkv* construct used for the experiments described in our paper (see below) is indeed downstream of UAS elements. However, the expression of the construct was not driven by a GAL4 line, but rather by enhancer piracy, and hence the promoter is unknown. The only information we are in a position to provide is that the line used is a jump of UAS-tkv* onto the TM3, Sb balancer chromosome. Please feel free to write to me for further clarifications. Regards, Pradeep Das >Dear Dr. Padgett, > >I am curating your paper for FlyBase: > >Das et al., 1998, Development 125(8): 1519-1528 > >and I have a question about the activated tkv* construct used in your >paper. > >Is this construct expressed using UAS regulatory sequences, and if >so, was expression driven using enGAL4 - as for the activated sax* >construct. If the tkv* construct is not expressed under the control >of UAS regulatory sequences, what promoter sequences drive its >expression ? > >I look forward to hearing from you, > >Gillian Millburn > Subject: Re: FlyBase query Dear Pradeep, thanks for your reply, it was very helpful. I would just like to confirm whether the UAS-tkv* construct used to make your tkv* enhancer piracy line is the construct that was described in: Lecuit et al., 1996, Nature 381(6581): 387--393 I would like to record the details of your tkv* enhancer piracy line as a personal communication to FlyBase from you. Is that OK ? Also, do you have a particular designation for the line, as this helps in naming the construct in FlyBase. Gillian > Subject: RE: FlyBase query Dear Gillian, the UAS-tkv* construct described in our paper was made in our lab, so while the nature of the mutation may be the same as the one in Lecuit et al. (and in several other papers), the transgenic line itself is unique. The designation we use for this line is 'SC143' (TM3, Sb, tkv*). Please cite personal communication from Seo-Hee Cho - he's the student in our lab that made the lines and characterised them. Hope that was helpful! Please feel free to email me for further clarifications. Regards, Pradeep > Subject: RE: FlyBase query Dear Pradeep, as you made the UAS-tkv* construct in your lab, I will make a new entry in FlyBase for it. Is the mutation Q199D, as described in the materials and methods of your paper for Ubi-tkv ? and is it in the pUAST vector ? As I've been writing to you, it seems fair if I cite the personal communication from both Seo-Hee Cho and you, and I'll record that the construct was made by him in the personal communication, is that OK ? Sorry to keep e-mailing you, and thanks for being so helpful ! Gillian > Subject: RE: FlyBase query dear gillian, >FlyBase for it. Is the mutation Q199D, as described in the materials and >methods of your paper for Ubi-tkv ? and is it in the pUAST vector ? yes to both. regards, pradeep
