We identified a lethal complementation group and named it double parked (it
blocks in two phases of the cell cycle- S and M). We found that a previously
identified lethal mutation with the isolation number l(2)51Ec is an allele of
dup. An adjacent female sterile, fs(2)PA77, complements l(2)51Ec, so these
were assumed to be two different genes. In fact, fs(2)PA77 is an allele of
dup, because it fails to complement our four lethal alleles which are stronger
than the l(2)51Ec mutation. In addition, we now have cloned the gene and
sequenced the PA77 mutation and shown it is causes an amino acid change in the
open reading frame.
Regards,
Terry Orr-Weaver