FlyBase Allele Report: Dmel\dup[PA77]

General Information

Symbol

Dmel\dup^PA77

Species

D. melanogaster

Name

FlyBase ID

FBal0031304

Feature type

allele

Associated gene

Dmel\dup

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Schupbach and Wieschaus, 1991, Underwood et al., 1990)

Progenitor genotype

Cytology

Description

Amino acid replacement: R342C.

(Whittaker et al., 2000)

Amino acid replacement in the open reading frame.

(Orr-Weaver, 1999.10.30)

Amino acid replacement: R?C.

(Whittaker, 1999.3.27)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:15,378,144..15,378,144 [-]

Nucleotide change:

C15378144T

Amino acid change:

R342C | dup-PA; R342C | dup-PB

Reported amino acid change:

R342C

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Whittaker et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Schupbach and Wieschaus, 1991)

lethal (with Df(2R)ED2426)

(Kahsai and Cook, 2018)

lethal (with Df(2R)Exel9015)

(Kahsai and Cook, 2018)

Phenotype Manifest In

follicle cell

(Whittaker et al., 2000)

follicle cell (with dup^a1)

(Whittaker et al., 2000)

mitotic cell cycle

(Whittaker et al., 2000)

mitotic cell cycle (with dup^a1)

(Whittaker et al., 2000)

nurse cell

(Whittaker et al., 2000)

nurse cell (with dup^a1)

(Whittaker et al., 2000)

Detailed Description

Statement

Reference

Genomic replication persists longer than normal in homozygous mutant females, being seen in stage 10A egg chambers. In ovaries from dup^PA77/dup^a1 female flies, incorporation of BrdU into the follicle cell genome is reduced. This reduction is most apparent at stage 9. No incorporation is seen at the chorion loci at any stage.

(Whittaker et al., 2000)

Eggs have reduced dorsal appendages and a thin, fragile cuticle: abnormalities in chorion gene amplification.

(Schupbach and Wieschaus, 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement