FlyBase Allele Report: Dmel\dup[a3]

General Information

Symbol

Dmel\dup^a3

Species

D. melanogaster

Name

FlyBase ID

FBal0095189

Feature type

allele

Associated gene

Dmel\dup

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: ?592term.

(Garner et al., 2001)

Amino acid replacement: Q592term.

(Whittaker et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:15,377,394..15,377,394 [-]

Nucleotide change:

C15377394T

Amino acid change:

Q592term | dup-PA; Q592term | dup-PB

Reported amino acid change:

Q592term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Garner et al., 2001, Whittaker et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Garner et al., 2001)

decreased occurrence of cell division | embryonic stage

(Shibutani et al., 2007)

lethal - all die during embryonic stage | recessive

(Whittaker, 1999.3.27)

some die during embryonic stage | recessive

(Whittaker, 1999.3.27)

Phenotype Manifest In

mitosis & nuclear chromosome

(Garner et al., 2001)

mitotic cell cycle

(Garner et al., 2001)

mitotic domain 1 | embryonic cycle 16

(Shibutani et al., 2007)

mitotic metaphase

(Garner et al., 2001)

Detailed Description

Statement

Reference

Homozygous embryos show weak BrdU incorporation during a prolonged and partial S phase of cycle 16.

(Shibutani et al., 2007)

Cells in homozygous dup^a1 mutant embryos enter mitosis 16 (M16) later than heterozygous controls. A partial S phase 16 is also seen. Once in M16 the cells are arrested remain in this state until at least stage 13, and mitotic cells are still visible at stage 15. Spindles in these mutants contain apparently functional centrosomes, chromosomes fail to align properly. Most chromosomes lie within the bipolar spindle but are scattered and not compacted into a metaphase plate. Severe alignment defects are seen in about 80% of mitotic cells.

(Garner et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

dup^a3 has abnormal mitotic cell cycle phenotype, suppressible by BubR1^k03113

(Garner et al., 2001)

dup^a3 has abnormal mitotic cell cycle phenotype, suppressible by mei-41^D3

(Garner et al., 2001)

Phenotype Manifest In

Suppressed by

Statement

Reference

dup^a3 has mitotic cell cycle phenotype, suppressible by BubR1^k03113

(Garner et al., 2001)

dup^a3 has mitotic cell cycle phenotype, suppressible by mei-41^D3

(Garner et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

In dup^a3, mei-41^D3 double mutants the mitosis 16 (M16) entry delay phenotype is absent. The addition of Bub1^k03113 to dup^a3 mutants suppresses the M16 arrest phenotype.

In dup^a3, mei-41^D3 double mutants the mitosis 16 (M16) entry delay phenotype is absent.

The addition of BubR1^k03113 to dup^a3 mutants suppresses the M16 arrest phenotype.

(Garner et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

dup^PA77

(Whittaker, 1999.3.27)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7276

dup^a3/CyO

Notes on Origin

Discoverer

Comments

dup^a3 fails to complement dup^l5.

(Whittaker, 1999.3.27)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

(Whittaker, 1999.3.27)

dup^a3

(Kondo and Hayashi, 2013, Shibutani et al., 2007)

speckled^a3

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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