FlyBase Allele Report: Dmel\BubR1[k03113]

(Liu et al., 2024, Li et al., 2010, Blower et al., 2006, Logarinho et al., 2004, Basu et al., 1999, Spradling et al., 1999, Roch et al., 1998, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Basto et al., 2000)

abnormal mitotic cell cycle | recessive

(Basu et al., 1999)

increased cell death | larval stage

(Basu et al., 1999)

increased mortality | nutrition conditional (with BubR1^MI01546)

(Liu et al., 2024)

lethal | recessive

(Basu et al., 1999, Spradling et al., 1999, Roch et al., 1998)

Phenotype Manifest In

adult fat body | nutrition conditional

(Liu et al., 2024)

fat body | nutrition conditional (with BubR1^MI01546)

(Liu et al., 2024)

imaginal disc

(Roch et al., 1998)

larval brain | larval stage

(Basto et al., 2000)

lipid droplet | nutrition conditional (with BubR1^MI01546)

(Liu et al., 2024)

mitotic anaphase

(Basu et al., 1999)

mitotic anaphase & nuclear chromosome

(Basu et al., 1999)

mitotic cell cycle

(Basto et al., 2000, Basu et al., 1999)

Detailed Description

Statement

Reference

The mitotic index of mutant larval brains is 0.65, compared to 1.02 in wild-type brains. Mutant larval brain neuroblasts treated with colchicine often do not arrest in prometaphase (which occurs in wild-type neuroblasts in response to colchicine), but instead enter an anaphase-like state with separated sister chromatids.

(Basto et al., 2000)

Homozygous lethality occurs mainly during the pupal stages, with most homozygotes surviving through the third larval instar stage. No mitotic abnormalities are seen in post-cellularisation homozygous embryos. Sister chromatids remain attached in only 32% of mitotic figures in homozygous third larval instar brains incubated for 1 hour with colchicine, indicating that the spindle checkpoint has often been bypassed. In brains that have not been treated with colchicine, the percentage of homozygous mitotic cells with separated sister chromatids is much higher, and the percentage of mitotic cells in prophase or prometaphase is much lower than in wild type. There is a threefold reduction in mitotic index. A significant number of anaphase figures in homozygous brains are aberrant, with three major kinds of abnormalities being seen at high frequency; firstly, chromosome bridges extend between the two separating groups of chromosomes in many anaphases, secondly, lagging chromatids remain behind at the position of the metaphase plate while other chromosomes have migrated to positions near the poles and thirdly, extensive chromosome fragmentation is seen in many anaphases. Mitotic cells in homozygous brains undergo vastly elevated levels of apoptosis compared to wild type.

(Basu et al., 1999)

Lethality occurs during third instar larval or pupal stages. Mutants exhibit visible disc abnormalities: very small or no discs. Clones cannot be induced in wild type discs. In tergites, clones with normal size and but lower than expected frequency are recovered. Clones cannot be recovered in the wing disc.

(Roch et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

BubR1^k03113 is a suppressor | partially of abnormal cell cycle phenotype of cid^T11-2/cid^T22-4

(Blower et al., 2006)

BubR1^k03113 is a suppressor | partially of abnormal mitotic cell cycle | embryonic stage phenotype of cid^T11-2/cid^T22-4

(Blower et al., 2006)

BubR1^k03113 is a suppressor of abnormal mitotic cell cycle phenotype of sub¹³¹

(Cesario et al., 2006)

BubR1^k03113 is a suppressor of abnormal mitotic cell cycle phenotype of dup^a3

(Garner et al., 2001)

BubR1^k03113 is a suppressor of abnormal mitotic cell cycle phenotype of asp^E3

(Basto et al., 2000)

Other

Statement

Reference

BubR1^k03113, sub¹³¹ has abnormal size | larval stage phenotype

(Cesario et al., 2006)

BubR1^k03113, sub¹³¹ has decreased cell number | larval stage phenotype

(Cesario et al., 2006)

BubR1^k03113, sub¹³¹ has decreased occurrence of cell division | larval stage phenotype

(Cesario et al., 2006)

Phenotype Manifest In

Suppressor of

Statement

Reference

BubR1^k03113 is a suppressor of brain | larval stage phenotype of sub¹³¹

(Cesario et al., 2006)

BubR1^k03113 is a suppressor of mitotic cell cycle phenotype of dup^a3

(Garner et al., 2001)

Other

Statement

Reference

BubR1^k03113, sub¹³¹ has brain | larval stage phenotype

(Cesario et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The presence of a BubR1^k03113 mutant background suppresses the increased mitotic index phenotype associated with cid^T11-2/cid^T22-4 mutants. The number of cells delayed in prophase and prometaphase also decreases dramatically in double mutants, whereas the number of cells in anaphase shows a corresponding increase and is greater than controls. BubR1^k03113 cid^T11-2/cid^T22-4 double mutants exhibit a mitotic index nearly double that of cid^T11-2/cid^T22-4 single mutants.

(Blower et al., 2006)

sub¹³¹, BubR1^k03113 double mutant larval brains show a lower percentage of cells in mitosis (0.65) compared to sub¹³¹ mutant brains (2.66) or wild-type brains (1.32).

sub¹³¹, BubR1^k03113 double mutant larval brains are slow growing, small, and contain fewer cells than BubR1^k03113 mutants.

(Cesario et al., 2006)

BubR1^k03113 suppresses the asp^E3 metaphase-arrest phenotype.

(Basto et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

BubR1^k06109

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10526

y¹ w^67c23; P{lacW}BubR1^k03113/CyO

Kyoto

102258

y¹ w^67c23 ; P{lacW}BubR1^k03113 / CyO

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

The P{lacW} insertions in BubR1^k03113 and BubR1^k06109 are at the same location within the BubR1 gene.

(Basu et al., 1999)

Precise excision of the P{lacW} insertion in BubR1^k03113 results in complete reversion of the lethality and associated mitotic defects seen in BubR1^k03113 homozygotes.

(Basu et al., 1999)

Complements: l(2)05315⁰⁵³¹⁵. Complements: vlc⁰⁷⁰²². Complements: l(2)09851⁰⁹⁸⁵¹. Complements: EcR^k04314. Complements: l(2)09851^k08138. Complements: l(2)k09848^k09848. Complements: Src42A^k10108.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

31/13

(Roch et al., 1998)

Bub1^k03113

BubR1^k03113

(Hasan et al., 2024, Liu et al., 2024, Das et al., 2016, Cesario et al., 2006)

bubR1²

(Logarinho et al., 2004)

bubR1^K03113

(Li et al., 2010)

bubR1^k03113

(Shinoda et al., 2023)

k03113

(Logarinho et al., 2004)

l(2)K03113

(Basu et al., 1999)

l(2)k02113

(Basto et al., 2000)

l(2)k03113

(Spradling et al., 1999)

l(2)k03113^k03113

(BDGP Project Members, 1994-1999)

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

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