FlyBase Allele Report: Dmel\BubR1[k06109]

(Perez-Mongiovi et al., 2005, Logarinho et al., 2004, Beaton, 2000.1.31, Basu et al., 1999, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

condensed chromosome

(Perez-Mongiovi et al., 2005)

condensed chromosome (with BubR1^Rev1)

(Perez-Mongiovi et al., 2005)

larval brain | larval stage

(Donaldson et al., 2001)

mitotic anaphase

(Basu et al., 1999)

mitotic anaphase & nuclear chromosome

(Basu et al., 1999)

mitotic cell cycle

(Basu et al., 1999)

neuroblast

(Perez-Mongiovi et al., 2005)

neuroblast (with BubR1^Rev1)

(Perez-Mongiovi et al., 2005)

spindle microtubule

(Wainman et al., 2009)

Detailed Description

Statement

Reference

BubR1^{KEN.T:Disc\RFP-mRFP},BubR1^k06109 mutant larval brains (third instar) do not show any growth defects and have normal number of neuroblast as well as the mitotic index, the rate of aneu- or polyploid brain cells is however increased compared to controls.

(Caous et al., 2015)

Mutant mitotic cells have aberrant spindles that are very short and only thinly populated by microtubules.

(Wainman et al., 2009)

Homozygous and BubR1^D1326N/BubR1^Rev1 females show elevated frequencies of exchange in the pericentromeric heterochromatin of the X chromosome.

(Malmanche et al., 2007)

Homozygous Bub1^k06109 mutants and Bub1^k06109/Bub1^Rev1 mutant neuroblasts exhibit a significant decrease in prophase-metaphase mitotic figures and a significant increase in sister chromatid separation.

Homozygous BubR1^k06109 mutants and BubR1^k06109/BubR1^Rev1 mutant neuroblasts exhibit a significant decrease in prophase-metaphase mitotic figures and a significant increase in sister chromatid separation.

(Perez-Mongiovi et al., 2005)

Homozygous lethality occurs mainly during the pupal stages, with most homozygotes surviving through the third larval instar stage. No mitotic abnormalities are seen in post-cellularisation homozygous embryos. The percentage of homozygous mitotic cells with separated sister chromatids is much higher, and the percentage of mitotic cells in prophase or prometaphase is much lower in homozygous third larval instar brains than in wild type. There is a reduction in mitotic index. A significant number of anaphase figures in homozygous brains are aberrant, with three major kinds of abnormalities being seen at high frequency; firstly, chromosome bridges extend between the two separating groups of chromosomes in many anaphases, secondly, lagging chromatids remain behind at the position of the metaphase plate while other chromosomes have migrated to positions near the poles and thirdly, extensive chromosome fragmentation is seen in many anaphases. Mitotic cells in homozygous brains undergo vastly elevated levels of apoptosis compared to wild type.

(Basu et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

BubR1^KEN.mRFP1, BubR1^k06109 has abnormal mitotic cell cycle | third instar larval stage phenotype, enhanceable by Sas-4^s2214/Sas-4^s2214

(Caous et al., 2015)

Enhancer of

Statement

Reference

BubR1^k06109/BubR1^KEN.mRFP1 is an enhancer of abnormal mitotic cell cycle | third instar larval stage phenotype of Sas-4^s2214

(Caous et al., 2015)

NOT Enhancer of

Statement

Reference

BubR1^k06109/BubR1^KEN.mRFP1 is a non-enhancer of neoplasia | third instar larval stage phenotype of aurA⁸⁸³⁹/aurA¹⁷⁹⁶¹

(Caous et al., 2015)

Suppressor of

Statement

Reference

BubR1^k06109 is a suppressor of abnormal mitotic cell cycle | recessive phenotype of polo^S132408

(Donaldson et al., 2001)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

BubR1^k06109/BubR1^KEN.mRFP1 is a non-enhancer of larval neuroblast | third instar larval stage | increased number phenotype of aurA⁸⁸³⁹/aurA¹⁷⁹⁶¹

(Caous et al., 2015)

Suppressor of

Statement

Reference

BubR1^k06109/BubR1^KEN.mRFP1 is a suppressor of larval neuroblast | increased number | third instar larval stage phenotype of Sas-4^s2214

(Caous et al., 2015)

BubR1^k06109 is a suppressor of mitotic cell cycle phenotype of polo^S132408

(Donaldson et al., 2001)

NOT Suppressor of

Statement

Reference

BubR1^k06109 is a non-suppressor of aster phenotype of polo^S132408

(Donaldson et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

The increased neuroblast number and mitotic index seen in aurA⁸⁸³⁹/aurA¹⁷⁹⁶¹ transheterozygote larvae is not enhanced by combination with BubR1^{KEN.T:Disc\RFP-mRFP},BubR1^k06109 (the mitotic index in the double mutants is even slightly decreased), whereas the rate of ploidy defects is increased compared to either of the single gene mutants.

The increased brain neuroblast (NB) number observed in Sas-4^s2214 mutant third instar larvae is fully suppressed by combination with BubR1^{KEN.T:Disc\RFP-mRFP},BubR1^k06109 (the NB number in the double mutants is even lower than in wild-type controls and the brains are also smaller), while the rate of ploidy defects is strongly increased compared to either of the single gene mutants.

(Caous et al., 2015)

Cells in polo^S132408 BubR1^k06109 double mutant larval brains have a mitotic index of 3.7, comparable to the wild-type value of 3.3 (polo^S132408 single mutants have a mitotic index of 25.0). The spindles still show the characteristic polo mutant defects at the poles (which are anastral). Sister chromatids already separated at metaphase and lagging chromatids at anaphase are seen (similar to those seen in BubR1 single mutants). 49.2% of mitotic figures are aneuploid or polyploid.

(Donaldson et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

BubR1^k03113

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

102444

y¹ w^67c23 ; P{lacW}BubR1^k06109 / CyO

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

After six generations of outcrossing the lethality continued to segragate with the P{lacW} insertion.

(Beaton, 2000.1.31)

The P{lacW} insertions in BubR1^k03113 and BubR1^k06109 are at the same location within the BubR1 gene.

(Basu et al., 1999)

Precise excision of the P{lacW} insertion in BubR1^k06109 results in complete reversion of the lethality and associated mitotic defects seen in BubR1^k06109 homozygotes.

(Basu et al., 1999)

Complements: l(2)09851⁰⁹⁸⁵¹. Complements: Src42A^k10108.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

Bub1^k06109

BubR1¹

(Rahmani et al., 2009)

BubR1^k06109

bub1^k6109

(Donaldson et al., 2001)

bubR1¹

(Landmann et al., 2020, Duranteau et al., 2016, Derive et al., 2015, Wainman et al., 2009, Malmanche et al., 2007, Perez-Mongiovi et al., 2005, Logarinho et al., 2004)

bubR1^k06109

(Shinoda et al., 2023)

k06109

(Logarinho et al., 2004)

l(2)K06109

(Basu et al., 1999)

l(2)k03113^k06109

(BDGP Project Members, 1994-1999)

l(2)k06109

(Spradling et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

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