Subject: Re: Helping FlyBase: UAS-al/AR143
Dear Rachel,
Yes, I made this construct, and yes it is as Gerard described it with
the >CD2,y+> flp-out cassette first described in the Jiang and Struhl,
'95 and Zecca et al., 1995 papers.
Best wishes.
Gary
'Rachel Drysdale (Genetics)' wrote:
>
> Dear Gary,
>
> I enclose below a correspondence between myself and Gerard Campbell. I
> am writing to you for confirmation that the flip-out cassette was
> indeed the the CD2, y+ cassette (which I see discussed in Zecca et al.,
> 1995) that Gerard mentions \- if so I have all the info I need.
>
> With best wishes,
>
> Rachel.
>
> \----------------------------------------------------------------------
> Rachel Drysdale, Ph.D.
>
> FlyBase (Cambridge),
> Department of Genetics,
> \----------------------------------------------------------------------
>
>
> Dear Rachel,
> It was made by Gary Struhl \- as far as I can remember the original
> construct is an al cDNA in pUAST with a flp out cassette between the UAS
> sequence and the cDNA (although I am not absolutely sure it is pUAST, but
> I don't think he uses anything else). The flp out cassette is y+ but is
> probably the CD2, y+ cassette (original ref Jiang and Struhl 95 or Zecca
> et al 95). Sorry I can't be more helpful, if this isn't good enough, you
> could e-mail Gary \- I think his name for the construct is AR143.
> Gerard
>
> 'Rachel Drysdale (Genetics)' wrote:
>
> > Dear Gerard,
> >
> > I am writing to you about a UAS-al construct that appears in
> > \*x FBrf0131382 == Pueyo et al., 2000, Development 127(24): 5391--5402
> > with you stated to be the originator.
> >
> > We have no record of this construct in FlyBase and must conclude that
> > it is new (i.e. has not yet been published) so I need to make a new
> > transposon construct for it and what not. It would be best if the data
> > I include in the records is accurate, Juan Pablo tells me it is a
> > flipped out version of a bigger construct (with a y+ cassette?) but
> > seems slightly unclear as to who made it.
> >
> > If you can fill me in on one or two details I would be most grateful.
> > It is likely that we have records for related constructs (a y+
> > appears in P{αTub84B(FRT.y+)hh.B} from Basler and Struhl, 1994,
> > Nature 368(6468): 208--214) so references to related publications would
> > help me get it all correct.
> >
> > Hope all is going well with you,
> >
> > With best wishes,
> >
> > Rachel.
> >
> > \----------------------------------------------------------------------
> > Rachel Drysdale, Ph.D.
> >
> > FlyBase (Cambridge),
> > \----------------------------------------------------------------------
>
> \--
> Gerard Campbell
> Department of Biological Sciences
> University of Pittsburgh