Subject: Re: Helping FlyBase: UAS-slp1
Dear Rachel,
The original transgene is a UAS>CD2,y+>slp1. Manfred used a form of the
transgene from which the >CD2,y+> was excised, so a residual FRT ('>')
remains between the UASpromoter and slp1 sequences. The vector is
pUAST. slp1 is a cDNA.
Hope this is what you need.
Best wishes.
Gary
'Rachel Drysdale (Genetics)' wrote:
>
> Dear Gary,
>
> I am curating this paper of Manfred's for FlyBase:
>
> FBrf0131347 == Lee and Frasch, 2000, Development 127(24): 5497--5508
>
> and have a question for you about the construct they refer to as
> UAS-slp1 (insertion on third chromosome, 'gift from Gary Struhl'). We
> have a record of a UAS-slp1 construct in FlyBase, FBtp0008246 ==
> P{UAS-slp1.R}, but it was published in
>
> FBrf0096157 == Riechmann et al., 1997, Development 124(15): 2915--2922
>
> and there is no obvious relation to you in this reference. Manfred has
> confirmed that this is not the same one as yours. So I need to make a
> new transposon for your UAS-slp1. Is it simply a slp1 cDNA in pUAST?
>
> I will curate your reply as a personal communication to FlyBase, so
> that this new transposon will be credited to you.
>
> All the best,
>
> Rachel.
>
> \----------------------------------------------------------------------
> Rachel Drysdale, Ph.D.
>
> FlyBase (Cambridge),
> Department of Genetics,
> \----------------------------------------------------------------------