Subject: Re: FlyBase Query (cy1076)
Dear Chihiro
We got the eve-tau-lacZ marker line from Chris Doe's lab (see the Materials
and Methods in Wolf et al. (1998) Development, 125, 3853-3863).
It is OK to quote my previous email as a personal communication to FlyBase.
Sorry that I did not make these issues clear in the paper and hope that I
have answered all your questions.
Cheers
Xiaohang
At  11:46  AM 4/12/01 \+0100, you wrote:
>Dear Xiaohang,
>
>Thanks for re-sending your reply, your original must have gone astray in
>the electronic ether.
>
>I have another question I should have asked you the first time, In
>figure 2 you show staining by and eve-tau-lacZ line for which you cite,
>Fujioka et al., 1999, Development 126(11): 2527--2538. In this paper
>there are a whole series of eve-lacZ line that are called in FlyBase:
>
>Ecol\lacZeve.F1 to Ecol\lacZeve.F40
>
>Is the line you used one of these?
>
>Also is it OK for me to make your last email a personal communication
>from you to FlyBase. The information you gave me about the 443/24 line
>is potentially useful to users and not stated anywhere else.
>
>Thanks
>Chihiro
>
> > Dear Chihiro
> > My apologies for not replying you earlier. I have to dig into my old
> > notebooks dated sometime 1994 to answer your questions. Yes, I made a
> > mistake and quoted wrongly in the paper the origin of the P1280 line. It
> > was from the Keith collections (Hungarian lines) and the original
> number is
> > 443/24. You should be able to convert it to a P-line starting with the
> > letter k (sorry I can not remember the rules for conversion now). The
> > second question is easier for me. We used the Scer\GAL4elav-C155
> line in
> > our experiment.
> > Hope these answers are useful.
> > Cheers
> > Xiaohang
> >
> > >Dear Dr Yang,
> > >
> > >I am currently curating your paper for Flybase:
> > >
> > >Bahri et al., 2001, Mech. Dev. 100(2): 291--301
> > >
> > >I have a couple of quick questions I was hoping you could answer for me.
> > >
> > >P1280
> > >-----
> > >You describe a lacZ enhancer trap P insertion you call P1280 citing
> > >Bloomington as your source. I cannot find a stock meeting this
> > >description with the identifier P1280. Do you have any other
> > >information about this line that might help me identify exactly which
> > >insertion you used? Do you have a Berkeley identifier (a five figure
> > >number possibly starting with a k, e.g.). Can you tell me which
> > >transposon was used to make this line? (Was it a P{PZ} line or a
> > >P{lacW} line).
> > >
> > >elav-GAL4
> > >---------
> > >In your paper you mention an elav-GAL4 line. There are two these in
> > >our records, could you tell me which one you used in your experiments?
> > >If you are not sure could you give me any information you can so I can
> > >work it out? For example, is it an enhancer trap line or a promoter
> > >fusion? Where did get the line from? Do you know of any papers that
> > >this line has been used in?
> > >
> > >Scer\GAL4elav.PLu
> > > Luo et al., 1994, Genes Dev. 8(15): 1787--1802
> > > P{GAL4-elav.L}
> > > GAL4 expression is driven by an elav promoter.
> > >
> > >Scer\GAL4elav-C155
> > > Rebay and Rubin, 1995, Cell 81(6): 857--866
> > > P{GawB}elavC155
> > > Enhancer trap
> > >
> > >Best wishes,
> > >
> > >Chihiro
> > >
> > >----------------------------------------------------------------------
> > >Chihiro Yamada.
> > >
> > >FlyBase (Cambridge),
> > >----------------------------------------------------------------------
> > >
> > >----- End Included Message \-----
> >