Subject: New Indy allele
IDENTIFICATION OF NEW Indyp115 ALLELE.
Bulgakova N., Trunova S., Omelyanchuk L.
Mutation Indyp115, caused by PlArB insertion in 75D region, was
initially isolated as cell-cycle related. Rescued genomic DNA was
cloned and sequenced.
Molecular data: P-target rescue placed this insertion of
PlArB-element in forward direction within 122 bp of putative
transcriptional start site. We found that EP(3)3044 is localized in
reversed orientation here.
Mutant phenotype: Homozygotes are fertile but have decreased viability.
The reporter b-galactosidase activity was detected in larval brain
(Fig1), eye-antennae imaginal disk (the staining was observed after the
front of morphogenetic furrow, Fig2), larval midgut (imaginal ring and
all the cells of the gut excluding gut brunches, Fig3), fat body
(Fig4). In adult flies staining was revealed in oenocytes, testis
(Fig5), and ovaries (region 2 in germaria, Fig6, some nurse cells,
stripped staining of the follicular cells in 14th stage old egg
chambers, Fig7). The determination of the lethal phase in
embryogenesis shows one lethal phase at 1-5 embryonic stages and a
second at 15-16 stages. Since the first lethal phase takes phase before
blastoderm stage where no zygotic genes are active we conclude that
p115 shows maternal effect on the viability of progeny. We suggest that
the early embryonic lethality may be due to wrong gene expression
detected by b-galactosidase staining in follicular cells.
Other information: Initially this mutation was described and studied as
male sterile (Kozlova A.V., Omelyanchuk L.V., Y-chromosomal factor
controls transctiption of autosomal fertility genes in Drosophila
melanogaster males. Ontogenez 1998 29(5): 366-372). The excision of
PlArB-element shows that the sterile phenotype is not connected with
insertion.With the use of meiotic crossingover we localized the male
sterile mutation in the ru \- h interval (61F7-66D10).
Bulgakova Natalia A.
Master Degree Student
Laboratory of Cell Cycle Genetics
Institute of Cytology and Genetics
Russian Academy of Sciences
Lavrentiev avenue, 10
Novosibirsk 630090
Russia