FlyBase Gene Report: Dmel\Indy

General Information

Symbol

Dmel\Indy

Species

D. melanogaster

Name

I'm not dead yet

Annotation Symbol

CG3979

Feature Type

protein_coding_gene

FlyBase ID

FBgn0036816

Gene Model Status

Current

Stock Availability

54 publicly available

Gene Summary

I'm not dead yet (Indy) encodes a plasma membrane transporter of Krebs cycle intermediates (citrate, succinate, fumarate, alpha-ketoglutarate) of the SLC13A family that is important in organismal intermediary metabolism. Mutations in Indy create a favorable metabolic state, similar to calorie restriction, and extend life span. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

75E1-75E2

Sequence location

3L:18,828,632..18,846,267 [-]

Recombination map

3-45

RefSeq locus

NT_037436 REGION:18828632..18846267

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables citrate transmembrane transporter activity

inferred from direct assay

(Inoue et al., 2002, Knauf et al., 2002)

enables pyruvate transmembrane transporter activity

inferred from direct assay

(Inoue et al., 2002)

enables succinate transmembrane transporter activity

inferred from direct assay

(Inoue et al., 2002, Knauf et al., 2002)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables citrate transmembrane transporter activity

inferred from biological aspect of ancestor with PANTHER:PTN000031937

(GO Reference Genome Project, 2011-)

enables succinate transmembrane transporter activity

inferred from biological aspect of ancestor with PANTHER:PTN000031937

(GO Reference Genome Project, 2011-)

enables transmembrane transporter activity

inferred from electronic annotation with InterPro:IPR001898

(InterPro Project Members, 2004-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in citrate transport

inferred from direct assay

(Inoue et al., 2002, Knauf et al., 2002)

involved_in determination of adult lifespan

inferred from direct assay

(Wang et al., 2009)

inferred from mutant phenotype

(Rogina et al., 2000)

involved_in pyruvate transport

inferred from direct assay

(Inoue et al., 2002)

involved_in regulation of triglyceride storage

inferred from direct assay

(Wang et al., 2009)

involved_in succinate transport

inferred from direct assay

(Inoue et al., 2002, Knauf et al., 2002)

NOT involved_in determination of adult lifespan

inferred from mutant phenotype

(Toivonen et al., 2007)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in citrate transport

inferred from biological aspect of ancestor with PANTHER:PTN000031937

(GO Reference Genome Project, 2011-)

involved_in succinate transport

inferred from biological aspect of ancestor with PANTHER:PTN000031937

(GO Reference Genome Project, 2011-)

involved_in transmembrane transport

inferred from biological aspect of ancestor with PANTHER:PTN000031793

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001898

(InterPro Project Members, 2004-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in plasma membrane

inferred from direct assay

(Knauf et al., 2002)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in membrane

inferred from electronic annotation with InterPro:IPR001898

(InterPro Project Members, 2004-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000031793

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

SLC13 FAMILY OF DICARBOXYLATE/CITRATE TRANSPORTERS

Protein Family (UniProt)

Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily. (Q9VVT2)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

SLC13 FAMILY OF DICARBOXYLATE/CITRATE TRANSPORTERS -: Solute carrier family 13 (SLC13) transmembrane transporters mediate the flux of dicarboxylate and citrate across the plasma membrane. These proteins are responsible for the transport of di- and tricarboxylic acid Krebs cycle intermediates such as succinate, citrate, and alpha-ketoglutarate. (Adapted from FBrf0156036 and FBrf0132448).

Protein Function (UniProtKB)

Cation-independent electroneutral transporter (not associated with membrane depolarization) of a variety of tricarboxylic and dicarboxylic acid-cycle intermediates. There is also small, but detectable, transport of monocarboxylics. Transport is through the epithelium of the gut and across the plasma membranes of organs involved in intermediary metabolism and storage. Affinity for substrates is citrate > succinate > pyruvate. Fumarate, a-ketoglutarate, and glutarate are also transported, but not lactate. Transport mechanism that is not coupled to Na(+), K(+), or Cl(-). Function is shown in Xenopus oocytes and human retinal pigment epithelial (HRPE) cell lines.

(UniProt, Q9VVT2)

Summary (Interactive Fly)

plasma membrane transporter of Krebs cycle intermediates - midgut - regulation of lifespan - regulation of mitochondrial function

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Indy for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VVT2)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

Comments on Gene Model

Transposon inserted in intron

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Indy-RA

FBtr0075083

NM_079426

2484

572

Indy-RB

FBtr0075081

NM_168778

2572

590

Indy-RC

FBtr0075082

NM_168779

2600

572

Indy-RD

FBtr0301857

NM_001169994

2581

572

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Indy-PA

FBpp0074850

63.6

572

8.55

FBpp0074848

65.6

590

8.55

FBpp0074849

63.6

572

8.55

FBpp0291071

63.6

572

8.55

E1JI19

NP_001163465

ACZ94736

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

572 aa isoforms: Indy-PA, Indy-PC, Indy-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Indy using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Indy_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.05

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | faint | ubiquitous

• cytoplasm | basal

Comment: apically cleared

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

primordial germ cell

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic/larval midgut

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in plasma membrane

inferred from direct assay

(Knauf et al., 2002)

Expression Deduced from Reporters

Reporter: P{PTT-GC}Indy^YC0017

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain subperineurial glial cell

(DeSalvo et al., 2014)

adult brain surface glial cell

(DeSalvo et al., 2014)

Reporter: PBac{754.P.FSVS-0}Indy^CPTI003049

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult antennal lobe

(Knowles-Barley, 2011.8.24)

adult brain cell body rind

(Knowles-Barley, 2011.8.24)

adult brain

Comment: low expression throughout adult brain

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Indy in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Indy

View exonic expression by tissue for Dmel\Indy

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0036816

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40049/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0036816

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0036816

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0036816

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3979

Flygut - An atlas of the Drosophila adult midgut

FBgn0036816

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Indy

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 52 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Indy

Transgenic constructs containing regulatory region of Indy

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | recessive

Indy^YC0017

partially lethal

Indy^P115

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Indy^GD2712

viable, with Scer\GAL4^Mef2.PR

Indy^GD2712

viable, with Scer\GAL4^pnr-MD237

Indy^GD2712

viable, with Scer\GAL4^tin.CΔ4

Indy^GD2712

Sterility

Allele

female fertile

Indy^P115

fertile

male fertile

Indy^P115

Other Phenotypes

Allele

decreased speed of aging | dominant

increased fecundity

increased fecundity | conditional

long lived

long lived | dominant

short lived | recessive

Indy³⁰²

some die during embryonic stage

Indy^P115

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (5)

Hsap\SLC13A2

14 of 14

Yes

Hsap\SLC13A5

13 of 14

Yes

Hsap\SLC13A3

12 of 14

Yes

Hsap\SLC13A1

11 of 14

Yes

Hsap\SLC13A4

11 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (5)

Rnor\Slc13a2

14 of 14

Yes

Rnor\Slc13a5

13 of 14

Yes

Rnor\Slc13a3

12 of 14

Yes

Rnor\Slc13a1

11 of 14

Yes

Rnor\Slc13a4

11 of 14

Yes

Mus musculus (laboratory mouse) (5)

Mmus\Slc13a2

14 of 14

Yes

Mmus\Slc13a5

13 of 14

Yes

Mmus\Slc13a1

11 of 14

Yes

Mmus\Slc13a3

11 of 14

Yes

Mmus\Slc13a4

11 of 14

Yes

Xenopus tropicalis (Western clawed frog) (11)

Xtro\slc13a3

9 of 13

Yes

Xtro\slc13a4l

8 of 13

Yes

Xtro\slc13a1

7 of 13

Yes

Xtro\slc13a5

6 of 13

Yes

Xtro\chst1

2 of 13

Xtro\slc13a2

2 of 13

Yes

Xtro\crybb3

1 of 13

Xtro\dusp29

1 of 13

Xtro\kiaa0753

1 of 13

Yes

Xtro\LOC100486174

1 of 13

Xtro\slc13a4

1 of 13

Yes

Danio rerio (Zebrafish) (6)

Drer\slc13a2

14 of 14

Yes

Drer\slc13a3

12 of 14

Yes

Drer\slc13a5a

12 of 14

Yes

Drer\slc13a5b

12 of 14

Yes

Drer\slc13a1

11 of 14

Yes

Drer\slc13a4

11 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\nac-1

13 of 14

Yes

Cele\nac-3

11 of 14

Yes

Cele\B0285.6

10 of 14

Yes

Cele\nac-2

8 of 14

Yes

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP007054

12 of 12

Yes

Agam\AgaP_AGAP007055

12 of 12

Yes

Arabidopsis thaliana (thale-cress) (4)

Atha\TDT

13 of 13

Yes

Atha\DiT1

1 of 13

Yes

Atha\DIT2.1

1 of 13

Yes

Atha\DiT2.2

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\PHO90

8 of 13

Yes

Scer\PHO91

8 of 13

Yes

Scer\PHO87

7 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPBC3B8.04c

7 of 12

Yes

Escherichia coli (enterobacterium) (4)

Ecol\yfbS

2 of 11

Yes

Ecol\citT

1 of 11

Yes

Ecol\ttdT

1 of 11

Yes

Ecol\ybhI

1 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Indy. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (8)

10 of 13

9 of 13

8 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

developmental and epileptic encephalopathy 25, with amelogenesis imperfecta

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 5 )

Allele

Disease

Evidence

References

Indy²⁰⁶

model of alcohol-related neurodevelopmental disorder

CEA

(Belhorma et al., 2021)

model of epilepsy

CEA

(Jeong et al., 2021)

Indy³⁰²

model of Kohlschutter-Tonz syndrome

CEA

(Jeong et al., 2021)

CEA with VGlut1¹

(Jeong et al., 2021)

CEA with Nmdar1^EP331

(Jeong et al., 2021)

CEA with Nmdar2^attP

(Jeong et al., 2021)

CEA with GluClα^glc1

(Jeong et al., 2021)

CEA with Idh3g^G9423

(Jeong et al., 2021)

Indy^GD2712

model of Kohlschutter-Tonz syndrome

CEA

(Jeong et al., 2021)

Indy^NIG.3979R

model of Kohlschutter-Tonz syndrome

CEA

(Jeong et al., 2021)

Indy^{T245M.B.UAS.Tag:V5}

model of Kohlschutter-Tonz syndrome

CEA

(Jeong et al., 2021)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

SLC13A5; solute carrier family 13 member 5

model of developmental and epileptic encephalopathy 25

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

Indy^GD2712

model of Kohlschutter-Tonz syndrome

is ameliorated by VGlut1^UAS.cDa

(Jeong et al., 2021)

Indy^NIG.3979R

model of Kohlschutter-Tonz syndrome

is ameliorated by VGlut1^UAS.cDa

(Jeong et al., 2021)

Indy^{T245M.B.UAS.Tag:V5}

model of Kohlschutter-Tonz syndrome

is ameliorated by VGlut1^UAS.cDa

(Jeong et al., 2021)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SLC13A2; solute carrier family 13 member 2

14 of 14

604148

SLC13A5; solute carrier family 13 member 5

13 of 14

608305

DEVELOPMENTAL AND EPILEPTIC ENCEPHALOPATHY 25 WITH AMELOGENESIS IMPERFECTA; DEE25

developmental and epileptic encephalopathy 25

SLC13A3; solute carrier family 13 member 3

12 of 14

606411

LEUKOENCEPHALOPATHY, ACUTE REVERSIBLE, WITH INCREASED URINARY ALPHA-KETOGLUTARATE; ARLIAK

SLC13A1; solute carrier family 13 member 1

11 of 14

606193

SLC13A4; solute carrier family 13 member 4

11 of 14

604309

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

65330

DroID - A comprehensive database of gene and protein interactions.

FBgn0036816

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Sodium-coupled sulphate, di- and tri-carboxylate transporters

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-45

Cytogenetic map

75E1-75E2

Sequence location

3L:18,828,632..18,846,267 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

75E1-75E2

Limits computationally determined from genome sequence between P{PZ}W⁰⁵⁰¹⁴ and P{lacW}l(3)j14E7^j14E7

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-45

(FlyBase, 2016)

3-42.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (54)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

603259

w¹¹¹⁸; P{VT033008-lexA}attP2

27901

w¹¹¹⁸; P{lacW}Indy²⁰⁶

27902

w¹¹¹⁸; P{lacW}Indy³⁰²

Korea

17437

P{VT033008-lexA}attP2

15938

P{VT033006-LexA::p65}JK22C

17561

P{VT033006-lexA::GADfl}attP2

17131

P{VT033006-lexA::GADfl}attP40

NIG-Fly

3979R-3

P{NIG.3979R}3

VDRC

v202281

P{VT033006-GAL4}attP2

v200242

P{VT033008-GAL4}attP2

v9981

w¹¹¹⁸; P{GD2712}v9981

v9982

w¹¹¹⁸; P{GD2712}v9982/TM3

More stocks available...

Genomic Clones (20)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (229)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0036816

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0036816

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Indy encodes a transporter that mediates transport of succinate and also citrate across the plasma membrane. The transport of succinate is not cation dependent. The transport of citrate is Na⁺-independent, but is markedly stimulated at pH6.0 compared to pH7.5.

(Knauf et al., 2002)

Area matching Drosophila EST AA441327. This EST has sequence similarity to Rat sodium dependant dicarboxylate transporter, AB001321.

(Davies, 2001.3.15)

Heterozygous Indy mutants show a near doubling of the average adult life-span without a decline in fertility or physical activity.

(Rogina et al., 2000)

Relationship to Other Genes

Source for database merge of

Source for merge of: Indy BEST:LP01220

(Rana, 2004.7.8)

Source for merge of: CG3979 anon- EST:fe3A7

(FlyBase, 1996-)

Source for merge of: Indy anon-WO0172774.70

(FlyBase, 1996-)

Additional comments

Source for merge of CG3979 anon- EST:fe3A7 was sequence comparison ( date:000605 ).

(FlyBase, 1996-)

Source for merge of Indy anon-WO0172774.70 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: Indy CG3979

(Rogina et al., 2000)

Nomenclature comments

Etymology

The gene is named "I'm not dead yet" based on a quip in the movie "Monty Python and the Holy Grail".

(Pennisi, 2000)

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

BEST:LP01220

CG3979

(Christis and Munro, 2012, Cammarato et al., 2011, Desalvo et al., 2011, Khanna et al., 2010, Fujikawa et al., 2009, Quinones-Coello, 2007, Hollien and Weissman, 2006)

INDY

(Kim et al., 2025, Jaramillo-Martinez et al., 2021, Staats et al., 2018, Carlson and Derr, 2007)

Indy

(Janssens et al., 2025, Chvilicek et al., 2024, Ku et al., 2023, Ceder and Fredriksson, 2022, Promislow et al., 2022, Fan et al., 2021, Tiwari and Mandal, 2021, Flatt, 2020, Parkhitko et al., 2020, Takai et al., 2020, Brunet Avalos et al., 2019, Huang et al., 2019, Hudry et al., 2019, Krittika and Yadav, 2019, Yan et al., 2019, Croset et al., 2018, Staats et al., 2018, Sui et al., 2018, Velentzas et al., 2018, Willmes et al., 2018, Maistrenko et al., 2016, Aradska et al., 2015, Bradley et al., 2015, Briga and Verhulst, 2015, Gene Disruption Project members, 2015-, Rogers and Rogina, 2015, Van Bortle et al., 2015, DeSalvo et al., 2014, Rogers and Rogina, 2014, Coelho et al., 2013, Levis et al., 2013-, Rogina and Helfand, 2013, Sen et al., 2013, Eleftherianos and Castillo, 2012, Iliadi et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Cho et al., 2011, Jones and Grotewiel, 2011, Kanda et al., 2011, Tatar, 2011, Bauer et al., 2010, Cook et al., 2010.2.12, Chittaranjan et al., 2009, Fujikawa et al., 2009, Neretti et al., 2009, Toivonen et al., 2009, Wang et al., 2009, Buszczak et al., 2007, Lai et al., 2007, Leandro et al., 2007, Quinones-Coello, 2007, Quinones-Coello et al., 2007, Toivonen et al., 2007, Zhan et al., 2007, Zhang et al., 2007, Hollien and Weissman, 2006, Hollien and Weissman, 2006, Bulgakova et al., 2004, Bulgakova et al., 2002)

anon-EST:fe3A7

anon-WO0172774.70

drIndy

(Inoue et al., 2002)

indy

(Khaerani et al., 2024, Sui and Dahmann, 2024, Burke et al., 2014, Coelho et al., 2013, Kenyon C., 2005, Boulianne, 2001)

Name Synonyms

I am not dead yet

(Aigaki et al., 2002)

I'm Not Dead Yet

(Staats et al., 2018)

I'm not dead yet

(Willmes et al., 2018, Maistrenko et al., 2016, Cammarato et al., 2011, Khanna et al., 2010, Carlson and Derr, 2007, Lai et al., 2007, Quinones-Coello et al., 2007, Zhan et al., 2007, Rogina et al., 2000)

Indy

(Titlow et al., 2023)

anon-fast-evolving-3A7

Secondary FlyBase IDs

FBgn0025199
FBgn0045781
FBgn0046362

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 84 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40049

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VVT2

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VVT2

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

E1JI19

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VVT2

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0036816

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40049/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0036816

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0036816

FlyMine - An integrated database for Drosophila genomics

FBgn0036816

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG3979

MARRVEL_MODEL - MARRVEL (model organism gene)

40049

PDB - An information portal to biological macromolecular structures

Linkouts

BioGRID - A database of protein and genetic interactions.

65330

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0036816

DroID - A comprehensive database of gene and protein interactions.

FBgn0036816

DRSC - Results frm RNAi screens

FBgn0036816

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Indy_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0036816

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0036816

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3979

Flygut - An atlas of the Drosophila adult midgut

FBgn0036816

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0036816

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Sodium-coupled sulphate, di- and tri-carboxylate transporters

References (149)

The following external sites may use different assemblies or annotations than FlyBase.

-1.05

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