FlyBase Allele Report: Dmel\Indy[206]

General Information

Symbol

Dmel\Indy²⁰⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0120792

Feature type

allele

Associated gene

Dmel\Indy

Associated Insertion(s)

P{lacW}Indy²⁰⁶

Carried in Construct

Also Known As

Indy206

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Rogina et al., 2000)

Progenitor genotype

Associated Insertion(s)

P{lacW}Indy²⁰⁶

Cytology

Description

P{lacW} insertion within a 1360 element that is present in the first intron of Indy, just upstream of the putative translational start site (the 1360 element is present in the same position in wild-type animals).

(Rogina et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol-related neurodevelopmental disorder

CEA

(Belhorma et al., 2021)

model of epilepsy

CEA

(Jeong et al., 2021)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased speed of aging | dominant

(Rogina et al., 2000)

fertile

(Rogina et al., 2000)

increased fecundity

(Rogina et al., 2000)

increased fecundity | conditional

(Marden et al., 2003)

long lived

(Wang et al., 2009, Marden et al., 2003)

Phenotype Manifest In

Detailed Description

Statement

Reference

Indy²⁰⁶ homozygotes demonstrate a significant life span extension.

There is a strong relationship between life span extension in Indy²⁰⁶ mutants and calorie restriction. At normal or high calorie conditions Indy²⁰⁶ heterozygote flies have a significant life span extension as compared to genetically matched controls. However, under low calorie conditions, Indy²⁰⁶ heterozygote mutant flies show minimal lifespan extension.

Long-lived Indy²⁰⁶ heterozygote flies living on high-calorie food are as sensitive to starvation stress as normal flies on a low-calorie food. Indy²⁰⁶ heterozygote long-lived flies on high-calorie food gain very little weight and thus have a similar lack of weight gain as normal control flies on low-calorie food. Indy²⁰⁶ heterozygote flies on high-calorie food store less lipids than normal flies living on high calorie-food conditions.

Indy²⁰⁶ flies that have been backcrossed to yw flies for more than 10 generations still display a significant life span extension as compared to genetically matched controls when grown on normal rich food. There is no life span extension when Indy²⁰⁶ is backcrossed into the w1118 background.

Indy²⁰⁶ long-lived heterozygote flies living on high-calorie food show an increase in spontaneous physical activity that matches the increased physical activity seen with wild-type flies on low-calorie food.

There is no difference in food intake between Indy²⁰⁶ heterozygotes and wild-type flies.

(Wang et al., 2009)

Heterozygous females and males do not show an extended lifespan (after correction for genetic and cytoplasmic background effects).

(Toivonen et al., 2007)

Homozygous mutants are long lived, but have normal metabolic rates and flight velocity. Mutant flies have higher fecundity than wild-type early in life when fed on high quality food, also having a higher total life-time egg production. When on low-calorie food though fecundity is significantly decreased.

(Marden et al., 2003)

Heterozygotes show an increase in mean life-span to 71 days (compared to 37 days for controls) at 25^oC, an extension in mean life-span of 92%. Extension of 1% maximal life-span is greater than 45%. At 18^oC, the increase in mean life-span in mutants approaches 100% and the increase in 1% maximum life-span approaches 50% compared to controls. Heterozygous males and females are normal or superior in fertility and fecundity. Heterozygous females show the same early peak of egg laying and fertility as control females but sustain the ability to produce larger numbers of offspring for a longer period of time.

(Rogina et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Indy[+]/Indy²⁰⁶, Scer\GAL4^DJ651 is a suppressor of short lived phenotype of Ctet\tetX^TNT-LC.UAS, Scer\GAL4^DJ651

(Bauer et al., 2004)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The reduced lifespan seen in males expressing Ctet\TeTxLC^TNT.Scer\UAS under the control of Scer\GAL4^DJ651 can be increased if the males also carry Indy²⁰⁶/+. Only a small increase in lifespan is seen in females expressing Ctet\TeTxLC^TNT.Scer\UAS under the control of Scer\GAL4^DJ651 and carrying Indy²⁰⁶/+.

(Bauer et al., 2004)

Xenogenetic Interactions

Statement

Reference

The reduced lifespan seen in males expressing Ctet\TeTxLC^TNT.Scer\UAS under the control of Scer\GAL4^DJ651 can be suppressed if the males also carry Indy²⁰⁶/+. Only a small increase in lifespan is seen in females expressing Ctet\TeTxLC^TNT.Scer\UAS under the control of Scer\GAL4^DJ651 and carrying Indy²⁰⁶/+.

(Bauer et al., 2004)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

27901

w¹¹¹⁸; P{lacW}Indy²⁰⁶

Notes on Origin

Discoverer

Comments

Excision of the P{lacW} element can revert the increased life-span phenotype.

(Rogina et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Indy206

(Toivonen et al., 2009, Wang et al., 2009, Rogina et al., 2000)

Indy²⁰⁶

(Belhorma et al., 2021, Jeong et al., 2021, Toivonen et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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