Subject: Isolation and Characterization of Df(2L)BSC5
Isolation and Characterization of Df(2L)BSC5
Jennifer Deal and Kevin Cook
Bloomington Stock Center, Indiana University
Df(2L)BSC5 was isolated as a P transposase-induced male recombination event
involving P{PZ}eIF-4a02439 and P{lacW}l(2)k09923k09923. The deletion
was isolated as a dp+-cn+ recombinant chromosome from the cross dpov1
cn1 bw1 females x P{PZ}eIF-4a02439 cn1/dpov1
P{lacW}l(2)k09923k09923; TMS, delta2-3 males. The Df(2L)BSC5 chromosome
retains the miniwhite marker from the P{lacW} construct. Polytene
chromosome squashes showed the breakpoints 26B1-2;26D1-2. Df(2L)BSC5
failed to complement P{lacW}Gef26k13720.