FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\PDZ-GEFk13720
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General Information
Symbol
Dmel\PDZ-GEFk13720
Species
D. melanogaster
Name
FlyBase ID
FBal0064121
Feature type
allele
Associated gene
Dmel\PDZ-GEF
Associated Insertion(s)
P{lacW}PDZ-GEFk13720
Carried in Construct
Also Known As
l(2)k13720, Gef261
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

hypomorphic allele - genetic evidence

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Boettner and Van Aelst, 2007)

amorphic allele - molecular evidence

(Lee et al., 2002)

hypomorphic allele - genetic evidence

(Wang et al., 2006)
Mutagen
P-element activity
(BDGP Project Members, 1994-1999)
Progenitor genotype
Associated Insertion(s)
P{lacW}PDZ-GEFk13720
Cytology
Description

Insertion of a P{lacW} element at position +46 of exon 0.

(Huelsmann et al., 2006)

P{lacW} insertion in the 5' upstream region of the Gef26 gene.

(Lee et al., 2002)
Allele components
Component
Use(s)
Inserted element
P{lacW}
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Mutations Mapped to the Genome
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      ectoderm | embryonic stage

      (Boettner and Van Aelst, 2007)

      ectoderm | embryonic stage (with Df(2L)BSC5)

      (Boettner and Van Aelst, 2007)

      embryo

      (Boettner and Van Aelst, 2007)

      embryo (with Df(2L)BSC5)

      (Boettner and Van Aelst, 2007)

      embryo (with PDZ-GEFC1)

      (Boettner and Van Aelst, 2007)

      embryo (with PDZ-GEFC2)

      (Boettner and Van Aelst, 2007)

      embryonic/first instar larval cuticle | embryonic stage

      (Boettner and Van Aelst, 2007)

      embryonic/first instar larval cuticle | embryonic stage (with Df(2L)BSC5)

      (Boettner and Van Aelst, 2007)

      epithelial cell | embryonic stage

      (Boettner and Van Aelst, 2007)

      eye

      (Singh et al., 2006, Lee et al., 2002)

      eye (with PDZ-GEF3)

      (Singh et al., 2006)

      eye (with PDZ-GEF4)

      (Singh et al., 2006)

      eye (with PDZ-GEFEP388)

      (Singh et al., 2006, Lee et al., 2002)

      eye | somatic clone

      (Lee et al., 2002)

      eye photoreceptor cell

      (Lee et al., 2002)

      eye photoreceptor cell (with PDZ-GEFEP388)

      (Lee et al., 2002)

      eye photoreceptor cell | somatic clone

      (Lee et al., 2002)

      ommatidium

      (Lee et al., 2002)

      ommatidium | somatic clone

      (Lee et al., 2002)

      ovariole

      (Lee et al., 2002)

      wing

      (Singh et al., 2006)

      wing (with PDZ-GEF3)

      (Singh et al., 2006)

      wing (with PDZ-GEF4)

      (Singh et al., 2006)

      wing (with PDZ-GEFEP388)

      (Singh et al., 2006)

      wing | somatic clone - Minute background

      (Boettner and Van Aelst, 2007)

      wing blade

      (Boettner and Van Aelst, 2007, Lee et al., 2002)

      wing disc | somatic clone - Minute background | third instar larval stage

      (Boettner and Van Aelst, 2007)

      wing hair | somatic clone - Minute background

      (Boettner and Van Aelst, 2007)

      wing margin

      (Lee et al., 2002)

      wing margin bristle | somatic clone - Minute background

      (Boettner and Van Aelst, 2007)
      Detailed Description
      Statement
      Reference

      Eye and wing phenotypes (small rough eyes, abnormal wings) and some degree of lethality are seen in various PDZ-GEF allelic combinations, with their relative strength in the following order: PDZ-GEF4/PDZ-GEF6 = PDZ-GEF4/PDZ-GEF5 > PDZ-GEF3/PDZ-GEF4 > PDZ-GEFk13720/PDZ-GEF3 = PDZ-GEFk13720/PDZ-GEF4 > PDZ-GEFEP388/PDZ-GEF3 > PDZ-GEFk13720/PDZ-GEFk13720 > PDZ-GEFk13720/PDZ-GEFEP388 > PDZ-GEFEP388/PDZ-GEFEP388.

      Many mutant females have three spermathecae instead of two, with different allelic combinations having different percentages of females with three spermathecae: PDZ-GEF3/PDZ-GEF3 22%, PDZ-GEF3/PDZ-GEF4 30%, PDZ-GEF4/PDZ-GEF5 59%, PDZ-GEF4/PDZ-GEF6 62%, PDZ-GEFk13720/PDZ-GEF4 % (all PDZ-GEFk13720/PDZ-GEF4, PDZ-GEFk13720/PDZ-GEF3 or PDZ-GEFEP388/PDZ-GEF3 females have two spermathecae).

      (Singh et al., 2006)

      The testes of Gef263/Gef26k13720 animals have normal numbers of germline stem cells.

      (Wang et al., 2006)

      Gef26k13720/Gef26EP388 flies have a slight rough eye phenotype, with some missing photoreceptor cells.

      Homozygous clones in the eye have a sunken and disarrayed morphology and show loss of many photoreceptor cells.

      Mosaic flies in which the eye is homozygous have a small and rough eye phenotype. Photoreceptor cell numbers per ommatidium are greatly reduced and some ommatidia are fused together.

      Homozygous adults escapers are recovered at 18oC. The wing edges are rolled downwards in these adults and have frayed margins. The eyes are small and rough compared to wild type and show loss of photoreceptor cells. The wing vein pattern is not affected in these escapers.

      Homozygous females are sterile, with no egg laying. Only a few homozygous ovarioles survive beyond stage 9 of oogenesis, with the rest showing significant degeneration and extensive apoptosis.

      (Lee et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      Enhancer of
      Other
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Enhancer of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      PDZ-GEFk13720/PDZ-GEF3 females have two spermathecae, but with the introduction of Rap1rvB1/+ or Rap2lSH0581/+ extra spermathecal capsule materials are observed in some females; PDZ-GEFk13720/PDZ-GEF3 wing and eye phenotypes are also enhanced.

      (Singh et al., 2006)

      The testes of Gef263/Gef26k13720 animals have normal numbers of germline stem cells but numbers are reduced significantly if the animals are also Rap2l1/+ or RrvB1/+.

      (Wang et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Complements

      PDZ-GEFrv-1

      (Lee et al., 2002)
      Partially rescued by

      PDZ-GEFk13720 is partially rescued by PDZ-GEFEGFP

      (Boettner and Van Aelst, 2007)

      PDZ-GEFk13720 is partially rescued by Scer\GAL4VP16.PDZ-GEF/PDZ-GEFUAS.cBa

      (Boettner and Van Aelst, 2007)
      Comments

      PDZ-GEF3 fails to complement the rough eye phenotype of PDZ-GEFk13720.

      (Singh et al., 2006)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer

      I. Kiss.

      (BDGP Project Members, 1994-1999)
      Comments
      Comments

      Excision of the P{lacW} insertion can revert the lethal phenotype.

      (Lee et al., 2002)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (9)
      References (12)