FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\PDZ-GEF3
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General Information
Symbol
Dmel\PDZ-GEF3
Species
D. melanogaster
Name
FlyBase ID
FBal0216661
Feature type
allele
Associated gene
Dmel\PDZ-GEF
Associated Insertion(s)
P{lacW}PDZ-GEF3
Carried in Construct
Also Known As
Gef263, l(2)SH1450
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Singh et al., 2006, Wang et al., 2006)
Mutagen
P-element activity
(Wang et al., 2006)
Progenitor genotype
Associated Insertion(s)
P{lacW}PDZ-GEF3
Cytology
Description

P{lacW}Gef263 is inserted in the 5' promoter sequence of the Gef26 gene.

(Wang et al., 2006)
Allele components
Component
Use(s)
Inserted element
P{lacW}
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      PDZ-GEF3/PDZ-GEF3 escapers have small rough eyes and abnormal wings. PDZ-GEF3/PDZ-GEF3 homozygous escapers or homozygous clones have ommatidia that lack various photoreceptors cells and display planar polarity defects. PDZ-GEF3/PDZ-GEF3 mutant wings often form ectopic veins in the middle inter-vein region or are fused with normal veins; wings may also have clumps on the surface (may be extra chaetae or sensilla).

      Eye and wing phenotypes (small rough eyes, abnormal wings) and some degree of lethality are seen in various PDZ-GEF allelic combinations, with their relative strength in the following order: PDZ-GEF4/PDZ-GEF6 = PDZ-GEF4/PDZ-GEF5 > PDZ-GEF3/PDZ-GEF4 > PDZ-GEFk13720/PDZ-GEF3 = PDZ-GEFk13720/PDZ-GEF4 > PDZ-GEFEP388/PDZ-GEF3 > PDZ-GEFk13720/PDZ-GEFk13720 > PDZ-GEFk13720/PDZ-GEFEP388 > PDZ-GEFEP388/PDZ-GEFEP388.

      Many mutant females have three spermathecae instead of two, with different allelic combinations having different percentages of females with three spermathecae: PDZ-GEF3/PDZ-GEF3 22%, PDZ-GEF3/PDZ-GEF4 30%, PDZ-GEF4/PDZ-GEF5 59%, PDZ-GEF4/PDZ-GEF6 62%, PDZ-GEFk13720/PDZ-GEF4 % (all PDZ-GEFk13720/PDZ-GEF4, PDZ-GEFk13720/PDZ-GEF3 or PDZ-GEFEP388/PDZ-GEF3 females have two spermathecae).

      (Singh et al., 2006)

      Gef263/Gef263 is semi-lethal. Escapers have small, rough eyes and abnormal wings.

      The number of male germline stem cells is dramatically reduced in the testes of Gef263/Gef264 animals compared to wild-type. The hub is not lost, but expression of some marker genes in the hub is. The number of cyst progenitor cells is also reduced in these testes.

      The testes of Gef263/Gef26k13720 animals have normal numbers of germline stem cells.

      Gef263 homozygous germline stem cell clones develop normally.

      During larval stages, male germline stem cells in Gef263/Gef264 animals become detached from the hub. Marker localisation data suggests that the adherens junctions between the hub cells and the surrounding stem cells are impaired.

      (Wang et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      PDZ-GEF3/PDZ-GEFk13720 has visible phenotype, enhanceable by Rap1[+]/Rap1rvB1

      (Singh et al., 2006)
      Suppressed by
      Statement
      Reference

      PDZ-GEF3/PDZ-GEF4 has visible phenotype, suppressible by Scer\GAL4da.PU/shgUAS.sgGFP

      (Singh et al., 2006)
      Other
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      PDZ-GEF3/PDZ-GEFk13720 has eye phenotype, enhanceable by Rap1[+]/Rap1rvB1

      (Singh et al., 2006)

      PDZ-GEF3/PDZ-GEFk13720 has wing phenotype, enhanceable by Rap1[+]/Rap1rvB1

      (Singh et al., 2006)
      Suppressed by
      Statement
      Reference

      PDZ-GEF3/PDZ-GEF4 has spermatheca | increased number phenotype, suppressible by Scer\GAL4da.PU/shgUAS.sgGFP

      (Singh et al., 2006)

      PDZ-GEF3/PDZ-GEF4 has eye phenotype, suppressible by Scer\GAL4da.PU/shgUAS.sgGFP

      (Singh et al., 2006)

      PDZ-GEF3/PDZ-GEF4 has wing phenotype, suppressible by Scer\GAL4da.PU/shgUAS.sgGFP

      (Singh et al., 2006)

      PDZ-GEF3/PDZ-GEF4 has cell-cell adherens junction & hub cell phenotype, suppressible by shgUAS.sgGFP/Scer\GAL4upd1.PU

      (Wang et al., 2006)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      PDZ-GEFk13720/PDZ-GEF3 females have two spermathecae, but with the introduction of Rap1rvB1/+ or Rap2lSH0581/+ extra spermathecal capsule materials are observed in some females; PDZ-GEFk13720/PDZ-GEF3 wing and eye phenotypes are also enhanced.

      Expression of shgScer\UAS.T:Avic\GFP-rs driven by Scer\GAL4da.PU almost fully suppresses the supernumerary spermathecae phenotype (as well as wing and eye phenotypes) in PDZ-GEF4/PDZ-GEF3 flies.

      (Singh et al., 2006)

      The testes of PDZ-GEF3/PDZ-GEFk13720 animals have normal numbers of germline stem cells but numbers are reduced significantly if the animals are also Rap2l1/+ or RrvB1/+.

      PDZ-GEF3/PDZ-GEF4 has no effect on the male germline stem cell over-proliferation phenotype due to upd1Scer\UAS.cCa; Scer\GAL4nos.UTR.T:Hsim\VP16.

      The reduction in germline stem cells seen in the testes of PDZ-GEF3/PDZ-GEF4 animals is significantly suppressed by Egfrf24/+.

      shgScer\UAS.T:Avic\GFP-rs; Scer\GAL4upd1.PU rescues the formation of adherens junctions between male germline stem cells and hub cells in the germinal proliferation center of PDZ-GEF4/PDZ-GEF3.

      (Wang et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      PDZ-GEF3/PDZ-GEF4 is rescued by PDZ-GEF+t11.6

      (Singh et al., 2006)
      Comments

      PDZ-GEF3 fails to complement the rough eye phenotype of PDZ-GEFk13720.

      PDZ-GEF+t11.6 rescues eye and wing phenotypes of PDZ-GEF3/PDZ-GEF4 flies.

      (Singh et al., 2006)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Isolated as part of P-element screen for lethals described in FBrf0159696.

      (Wang et al., 2006)
      Comments
      Comments

      Excision of the insertion can revert the lethal phenotype.

      (Singh et al., 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Gef263
      (Michel et al., 2011, Boettner and Van Aelst, 2007, Singh et al., 2006)
      PDZ-GEF3
      (Sawant et al., 2018)
      l(2)SH1450
      (Singh et al., 2006, Wang et al., 2006)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)