FlyBase Allele Report: Dmel\PDZ-GEF[4]

General Information

Symbol

Dmel\PDZ-GEF⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0216662

Feature type

allele

Associated gene

Dmel\PDZ-GEF

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Singh et al., 2006, Wang et al., 2006)

Mutagen

ethyl methanesulfonate

(Wang et al., 2006)

Progenitor genotype

Cytology

Description

A deletion of about 900 bp in the 5' promoter sequence of the Gef26 gene.

(Wang et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | spermatogenesis (with PDZ-GEF³)

(Wang et al., 2006)

decreased cell number | spermatogenesis (with PDZ-GEF⁵)

(Wang et al., 2006)

decreased cell number | spermatogenesis (with PDZ-GEF⁶)

(Wang et al., 2006)

partially lethal (with PDZ-GEF³)

(Singh et al., 2006)

partially lethal (with PDZ-GEF⁵)

(Singh et al., 2006)

partially lethal (with PDZ-GEF⁶)

(Singh et al., 2006)

partially lethal (with PDZ-GEF^k13720)

(Singh et al., 2006)

sterile (with PDZ-GEF³)

(Wang et al., 2006)

sterile (with PDZ-GEF⁵)

(Wang et al., 2006)

sterile (with PDZ-GEF⁶)

(Wang et al., 2006)

visible (with PDZ-GEF³)

(Singh et al., 2006)

visible (with PDZ-GEF⁵)

(Singh et al., 2006)

visible (with PDZ-GEF⁶)

(Singh et al., 2006)

visible (with PDZ-GEF^k13720)

(Singh et al., 2006)

Phenotype Manifest In

cell-cell adherens junction & hub cell (with PDZ-GEF³)

(Wang et al., 2006)

cyst progenitor cell (with PDZ-GEF³)

(Wang et al., 2006)

eye (with PDZ-GEF³)

(Singh et al., 2006)

eye (with PDZ-GEF⁵)

(Singh et al., 2006)

eye (with PDZ-GEF⁶)

(Singh et al., 2006)

eye (with PDZ-GEF^k13720)

(Singh et al., 2006)

germinal proliferation center (with PDZ-GEF³)

(Wang et al., 2006)

hub cell (with PDZ-GEF³)

(Wang et al., 2006)

male germline stem cell (with PDZ-GEF³)

(Wang et al., 2006)

male germline stem cell (with PDZ-GEF⁵)

(Wang et al., 2006)

male germline stem cell (with PDZ-GEF⁶)

(Wang et al., 2006)

male germline stem cell | third instar larval stage (with PDZ-GEF³)

(Wang et al., 2006)

spermatheca | increased number (with PDZ-GEF³)

(Singh et al., 2006)

spermatheca | increased number (with PDZ-GEF⁵)

(Singh et al., 2006)

spermatheca | increased number (with PDZ-GEF⁶)

(Singh et al., 2006)

wing (with PDZ-GEF³)

(Singh et al., 2006)

wing (with PDZ-GEF⁵)

(Singh et al., 2006)

wing (with PDZ-GEF⁶)

(Singh et al., 2006)

wing (with PDZ-GEF^k13720)

(Singh et al., 2006)

Detailed Description

Statement

Reference

Eye and wing phenotypes (small rough eyes, abnormal wings) and some degree of lethality are seen in various PDZ-GEF allelic combinations, with their relative strength in the following order: PDZ-GEF⁴/PDZ-GEF⁶ = PDZ-GEF⁴/PDZ-GEF⁵ > PDZ-GEF³/PDZ-GEF⁴ > PDZ-GEF^k13720/PDZ-GEF³ = PDZ-GEF^k13720/PDZ-GEF⁴ > PDZ-GEF^EP388/PDZ-GEF³ > PDZ-GEF^k13720/PDZ-GEF^k13720 > PDZ-GEF^k13720/PDZ-GEF^EP388 > PDZ-GEF^EP388/PDZ-GEF^EP388.

Many mutant females have three spermathecae instead of two, with different allelic combinations having different percentages of females with three spermathecae: PDZ-GEF³/PDZ-GEF³ 22%, PDZ-GEF³/PDZ-GEF⁴ 30%, PDZ-GEF⁴/PDZ-GEF⁵ 59%, PDZ-GEF⁴/PDZ-GEF⁶ 62%, PDZ-GEF^k13720/PDZ-GEF⁴ % (all PDZ-GEF^k13720/PDZ-GEF⁴, PDZ-GEF^k13720/PDZ-GEF³ or PDZ-GEF^EP388/PDZ-GEF³ females have two spermathecae).

(Singh et al., 2006)

The number of male germline stem cells is dramatically reduced in the testes of Gef26³/Gef26⁴ animals compared to wild-type. In Gef26⁴/Gef26⁵ or Gef26⁴/Gef26⁶ the reduction is even more dramatic, with some testes lacking germ cells altogether. The secondary spermatogonia (identifiable by their branched fusomes) move to the tip.

In Gef26³/Gef26⁴ testes, the hub is not lost, but expression of some marker genes in the hub is. The number of cyst progenitor cells is also reduced in these testes.

During larval stages, male germline stem cells in Gef26³/Gef26⁴ animals become detached from the hub. Marker localisation data suggests that the adherens junctions between the hub cells and the surrounding stem cells are impaired.

(Wang et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

PDZ-GEF³/PDZ-GEF⁴ has visible phenotype, suppressible by Scer\GAL4^da.PU/shg^UAS.sgGFP

(Singh et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

PDZ-GEF³/PDZ-GEF⁴ has spermatheca | increased number phenotype, suppressible by Scer\GAL4^da.PU/shg^UAS.sgGFP

(Singh et al., 2006)

PDZ-GEF³/PDZ-GEF⁴ has eye phenotype, suppressible by Scer\GAL4^da.PU/shg^UAS.sgGFP

(Singh et al., 2006)

PDZ-GEF³/PDZ-GEF⁴ has wing phenotype, suppressible by Scer\GAL4^da.PU/shg^UAS.sgGFP

(Singh et al., 2006)

PDZ-GEF³/PDZ-GEF⁴ has cell-cell adherens junction & hub cell phenotype, suppressible by shg^UAS.sgGFP/Scer\GAL4^upd1.PU

(Wang et al., 2006)

PDZ-GEF⁶/PDZ-GEF⁴ has cell-cell adherens junction & hub cell phenotype, suppressible by Rap1^{V12.UAS.Tag:MYC}/Scer\GAL4^upd1.PU

(Wang et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of shg^{Scer\UAS.T:Avic\GFP-rs} driven by Scer\GAL4^da.PU almost fully suppresses the supernumerary spermathecae phenotype (as well as wing and eye phenotypes) in PDZ-GEF⁴/PDZ-GEF³ flies.

(Singh et al., 2006)

PDZ-GEF³/PDZ-GEF⁴ has no effect on the male germline stem cell over-proliferation phenotype due to upd1^Scer\UAS.cCa; Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

The reduction in germline stem cells seen in the testes of PDZ-GEF³/PDZ-GEF⁴ animals is significantly suppressed by Egfr^f24/+.

R^{V12.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^upd1.PU rescues the formation of adherens junctions between male germline stem cells and hub cells in the germinal proliferation center of PDZ-GEF⁴/PDZ-GEF⁶ animals. The same phenotype in PDZ-GEF⁴/PDZ-GEF³ animals is rescued by shg^{Scer\UAS.T:Avic\GFP-rs}; Scer\GAL4^upd1.PU.

(Wang et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

PDZ-GEF³/PDZ-GEF⁴ is rescued by PDZ-GEF^+t11.6

(Singh et al., 2006)

Partially rescued by

PDZ-GEF⁵/PDZ-GEF⁴ is partially rescued by PDZ-GEF^+t11.6

(Singh et al., 2006)

Comments

PDZ-GEF^+t11.6 rescues eye and wing phenotypes of PDZ-GEF³/PDZ-GEF⁴, PDZ-GEF⁴/PDZ-GEF⁵ or PDZ-GEF⁴/PDZ-GEF⁶ flies. PDZ-GEF^+t11.6 partially rescues the supernumerary spermathecae phenotype in PDZ-GEF⁴/PDZ-GEF⁵ flies.

(Singh et al., 2006)

Germline stem cell loss in the testes of Gef26⁴/Gef26⁶ animals is rescued by Gef26^+t11.6.

(Wang et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Gef26⁴

(Singh et al., 2006)

PDZ-GEF⁴

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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