Subject: more data
Dear Rachel,
This work has been done with mus106D1,
The following Df delete/disrupt mus106:
Df(1)g-1
and we tested two other Df that does not delete/disrupt mus106:
Df(1)N105
Df(1)C246
I join to this e-mail a table with the relevant numbers. Treatments
as described in Sibon et al., 1999.
As a consequence this work maps mus106 in 12A3- 10:12B7 , since it has
been previously mapped by recombination to the left of g. The gene is
thus located between up and g.
We have been testing different P elements for complementation, and
they all complement the MMS sensitivity.
EP(X)0385
EP(X)1396
EP(X)0395
EP(X)1329
EP(X)1353
We have also made sure that mus101 that is in the same interval is
not allelic to mus106.
Sincerely yours,
Anne Laurencon
| | |
| MMS-0.08% | MMS-0.1% | Control
crosses |106 sibs | 106 sibs | 106 sibs
__________________________|___________|___________|__________
w mus106/FM7c \* w mus106 | 9 24 | 13 26 | 120 64
| | |
|106/Df sibs|106/Df sibs|106/Df sibs
__________________________|___________|___________|__________
Df(1)N105/FM6 \* w mus106 | 33 66 | 22 37 | 69 150
| | |
Df(1)C246/FM6 \* w mus106 | 433 82 | 38 33 | 53 88
| | |
Df(1)g-1, f B/In(1)AM | | |
\* w mus106 | 0 37 | 0 63 | 94 152
__________________________|___________|___________|__________
|106/EP sibs|106/EP sibs|106/EP sibs
__________________________|___________|___________|__________
w mus106/FM7c \* w EP(X)1396| 25 17 | 25 5 | 91 187
| | |
w mus106/FM7c \* w EP(X)1353| 13 7 | 9 3 | 79 164
| | |
w mus106/FM7c \* w EP(X)1329| 74 48 | 26 3 | 91 191
| | |
w mus106/FM7c \* w EP(X)0395| 90 86 | 42 8 | 67 136
| | |
w mus106/FM7c \* w EP(X)0385| 33 23 | 27 8 | 74 183
__________________________|___________|___________|__________
|106/101sibs|106/101sibs|106/101sibs
__________________________|___________|___________|__________
w mus106D1/FM7c | 18 13 | 35 20 | 75 165
\* w mus101D2 | | |
__________________________|___________|___________|__________