Subject: Re: FlyBase Query (cy1375)
The BamHI site was introduced in ccgcaggaTccaaatccc, 64bp
downstream of the tatabox of YP1. So it's an YP1 promoter fusion.
..
Jean-Marc
\-------------------------
>Hi,
>
>Thanks for your reply. I just have one point I need to clarify.
>
>So you have used a the piece of genomic DNA from between the YP1 and
>YP2 genes. Does this fragment have exon 1 of just YP1 or both? If it
>has both, which gene was fused to the GAL4 i.e. which gene is the BamHI
>site located in?
>
> > A BamHI site was introduced 64bp downstream of the tatabox of the YP1
> > gene (LOCUS DMYOLK1 1677 bp DNA INV
> > 07-NOV-1997
> > DEFINITION Drosophila intergenic spacer and exon 1 from YPI and
>YPII gene for
> > yolk protein. ACCESSION X01524 M11170, prom Yolk-prot1).
..
>Chihiro.
Subject: Re: FlyBase Query (cy1375)
>Hi Chihiro,
Sorry for the delay, but you know how it is...The Gal4 line
was constructed here.
A BamHI site was introduced 64bp downstream of the tatabox of the YP1
gene (LOCUS DMYOLK1 1677 bp DNA INV
07-NOV-1997
DEFINITION Drosophila intergenic spacer and exon 1 from YPI and YPII gene for
yolk protein. ACCESSION X01524 M11170, prom Yolk-prot1). This
Hind3-BamHI(new) fg. was fused to BamHI site 5' of the Gal4 coding
region of pGATB (Brand & Perrimon) . The whole fusion was cloned into
pCasPer as an EcoRI-NotI 4192bp fg. and injected.
The sequence of the yolk casper vector is available. I can send it to
you or people can ask it from me.
The expression is fat body in female only which is what I was
looking for. By the way, I received a lot of requests for this line
and I'm still amplifying it before giving it away. I think that I
will send it to Bloomington.
Hope this helps...
Don't hesitate to contact me if needed..
Take care
Jean-Marc
\--------------------
>Hiya Jean Marc,
>
>I'm currently curating:
>
>Vidal et al., 2001, Genes Dev. 15(15): 1900--1912
>
>In this paper Vidal et al. use a GAL4 line they call yolk-GAL4, for
>which they cite you as the source. We don't have a GAL4 line with that
>name. Can you identify this line? Has it been published before? If
>so under what name? If not, can you give me some details about this
>line. For example; Is it an enhancer trap? What gene is it inserted
>into? Any new information you give us will be curated as a personal
>communication from you to FlyBase.
>
>Thanks
>
>Chihiro
>
>----------------------------------------------------------------------
>Chihiro Yamada.
>
>FlyBase (Cambridge),
>----------------------------------------------------------------------
Jean-Marc REICHHART
UPR 9022 CNRS IBMC
15, rue Rene Descartes
67084 \- Strasbourg Cedex
FRANCE