Subject: Re: FlyBase query
Hi!
1) UASDll \- the one I used does not come from any of the sources you
mentioned. It's original name was y w hs-FLP122;UAS>CD2 y+>Dll, but it
had the CD2 y+ cassette FLPed out. I think I got it from Richard Mann
(Columbia), but I have not seen him ever publish this line.
2) UASdac21M5: the reference is Shen and Mardon, 1997 (same reference as
for UASdac7c4). It is a UASdac on the 3rd while UASdac7c4 is on the 2nd
chromosome. ..
Hope this helps.
Cordelia
'Gillian Millburn (Genetics)' wrote:
> Dear Dr. Rauskolb,
>
> I am curating your paper for FlyBase:
>
> Rauskolb, 2001, Development 128(22): 4511--4521
>
> and I have a couple of questions.
>
> 1. UASDll
>
> FlyBase has a record of 3 UAS-Dll constructs:
>
> a. 'construct a of Basler'
>
> described in:
>
> Dong et al., 2000, Development 127(2): 209--216
>
> b. 'construct a of Gorfinkiel'
>
> described in:
>
> Gorfinkiel et al., 1997, Genes Dev. 11(17): 2259--2271
>
> c. 'construct a of Wu'
>
> described in:
>
> Wu and Cohen, 1999, Development 126(1): 109--117
>
> do you know which is the one you used ? (if not, who did you get the
> construct from and do you know a reference where it has been described)
>
> 2. UASdac21M5
>
> Is this UAS-dac construct the same as the 'UASdac7c4' construct you
> also mention in the Materials and Methods or are they different
> constructs ?
>
> Could you tell me who you got the 'UASdac21M5' construct from and a
> reference where it has been used, so that I can work out which of the
> UAS-dac constructs in FlyBase it corresponds to,
>
> I look forward to hearing from you,
>
> Gillian
>
> \--------------------------------------------------------------
> Gillian Millburn.
>
> FlyBase (Cambridge),
> \--------------------------------------------------------------