Subject: l(2)k05909
Hi,
I have some unpublished results which may be of interest to FlyBase and
the Drosophila research community. Our genetic analysis of l(2)k05909
suggests that it is an allele of synaptotagmin (syt). In the attachement
of this e-mail I am sending a txt
file describing our experimental results.
Best regards,
\--
Raymund Stefancsik, Ph.D.
Cutaneous Biology Research Center
Massachusetts General Hospital/Harvard Medical School
Building 149, 13th Street
Charlestown, MA 02129
USA
Genetic analysis of two insertions, l(2)k05909 and EP(2)1039,
located close to Alp23B
Our laboratory is interested in TGF-beta signal transduction,
so we tested two P element insertions in the proximity of
Alp23B (Activin Like Protein at 23B, FBgn0031461) as
potential alleles of this gene. The results are summarized below.
A) l(2)k05909 is lethal in trans to sytT77 or
deficiencies uncovering syt (see Table 1). The only lethal
mutation on the sytT77 chromosome is in syt, because it can
be rescued by a synaptotagmin cDNA transgene (sytelav.PD.),
when homozygous or in trans to sytN6 (Littleton et al.,
1994, Calcium dependence of neurotransmitter release and rate
of spontaneous vesicle fusions are altered in Drosophila
synaptotagmin mutants. Proc. Natl. Acad. Sci. USA 91(23):
10888--10892 <up>FBrf0077252</up>). From the lack of
complementation between l(2)k05909 and sytT77 we conclude
that l(2)k05909 (FBgn0022151) is an allele of synaptotagmin
(syt, FBgn0004242).
B) The sequence recovered from the 3' end of P element
EP(2)1039 (FBti0010740, GenBank \#AQ254821) maps very close to
the upstream region of the Alp23B transcribed region.
EP(2)1039 is homozygous viable (see Table 1) with no apparent
abnormalities. It is not an allele of Cy (FBgn0000403), which
also maps to this region.
_____________________________________________________
TABLE 1:
l(2)k05909 EP(2)1039 Cy1
Df(2L)C144 \- \+ \-
Df(2L)JS31 \- \+ \-
Df(2L)N6 \- \+ \+
Df(2)JS17 \+ \+ \+
sytT77 \- nd \+
Cy67.3 \+ \+ \-
EP(2)1039 \+ \+ \+
_____________________________________________________
TABLE LEGEND:
\+: viable in trans (with no visible abnormalities)
\-: lethal in trans
nd: not determined
FLY STOCKS USED:
y1 w67c23; P{w+mC=lacW}l(2)k05909k05909/CyO (SOURCE:
Bloomington Stock Center, BL-10586)
Df(2L)C144, dppd-ho ed1/In(2LR)Gla, wgGla-1 Bc1
EgfrE1 (SOURCE: Bloomington Stock Center, BL-90)
Dp(2;1)JS13, z1 w11E4; Df(2L)JS31, dppd-ho/CyO CyO
(SOURCE: Bloomington Stock Center, BL-1581)
y1 w*; Df(2L)N6, P{w+mC=lacW}B8-2-30, sytN6/CyO
(SOURCE: Bloomington Stock Center, BL-3910)
Df(2L)JS17, dppd-ho/CyO, P{ry+t7.2=en1}wgen11 (SOURCE:
Bloomington Stock Center, BL-1567)
y1 w*; P{w+mC=lacW}sytT77/CyO (SOURCE: Bloomington
Stock Center, BL-4377)
EP(2)1039/CyO (balancer is a guess R.S.) (SOURCE: Szeged
Stock Center)
Cy67.3 dppd*/In(2LR)Gla, wgGla-1 (SOURCE: Bloomington
Stock Center, BL-3918)
\-
Raymund Stefancsik, Ph.D.
Cutaneous Biology Research Center
Massachusetts General Hospital/Harvard Medical School
Building 149, 13th Street
Charlestown, MA 02129
USA