In response to my query:
Subject: Curation query
I am currently curating your recent paper (Eissenberg et al., 2002, PNAS, USA
99(15): 9894--9899) for FlyBase. Would it be possible for you to help me with
clarify the following issue:
The numbering of the Su(Tpl) sequence in figure 2, doesn't appear to match
that used in the text for decribing the the changes seen in particular alleles.
e.g.
Su(Tpl)17: nonsense mutation \- encodes a C terminally truncated protein of
789 aa.
In Su(Tpl)S-192 missense mutations: R917G & D919N in the text, In fig are
R926G & D928N
Which numbers should I use in curating the leisions?'
Joel Eissenberg replied:
'Subject: Re: Curation query
... the numbers running down the left-hand side of the sequence should be,
from top
to bottom: 1, 89, 177, 265, 353, 441, 529, 617, 705, 793, 881, and 969. In
addition, the position of the tick in the Su(Tpl)17 mutation is shifted to
the right by one amino acid. Now, this makes mutation in Su(Tpl)17 a
Q789stop mutation, and the protein would be 788 a.a. in length.
For Su(Tpl)S-192, this would make the mutations you asked about R916G and
D918N. In addition, the third mutation in this allele is S660L, which is
correctly stated in the text. The text
doesn't state the position of the XS-706 substitution, but it is G501S.
The annotation of the S1 and S2 alleles are unchanged.'