The following information accompanied stocks donated to the Bloomington Stock Center by Mariann Bienz, MRC Laboratory of Molecular Biology (9/03).
Imprecise w- excisions and lethal w+ hops were generated from P{wAR}4-95, an insertion near Apc2. Two lethal insertions in the 5' UTR of jaguar (P{wAR}jar2095 and P{wAR}jar1646) were isolated. Four of the imprecise excisions were alleles of l(3)95Ea including the allele l(3)95Eaw1. Two of the imprecise excisions were the deletions Df(3R)w5 and Df(3R)w6.
Df(3R)w5 extends to the right from the P{wAR}4-95 insertion site.
Df(3R)w6 extends to the left from the P{wAR}4-95 insertion site.
In a screen for lethal mutations in 95EF, an isogenized P{neoFRT}82B P{piM}87E stock was treated with 45 mM EMS. Lethals were selected based on noncomplementation with Df(3R)crb87-4 and complementation with Df(3R)crb87-5. Ten complementation groups were recovered, each of which was represented by at least two alleles. Df(3R)w5 and Df(3R)w6 were used to subdivide the loci: l(3)95Ea, l(3)95Eb, l(3)95Ec, l(3)95Ed and l(3)95Ef fall within Df(3R)w6, and l(3)95EFa, l(3)95EFb, l(3)95EFc, l(3)95EFd and jar fall within Df(3R)w5. Alleles of these complementation groups include those listed below along with phenotypic information.
l(3)95Ea4437 Clones with rough eyes, vein defects and wing nicks
l(3)95Ea5383 Clones with rough eyes and missing notum bristles
l(3)95Eb1117 Normal embryos and clones
l(3)95Eb3627 Normal embryos and clones
l(3)95Ec149 Normal embryos. clones with rough eyes and vein defects
l(3)95Ec455 Abnormal embryos. Clones with rough eyes, wing nicks and vein and bristle defects.
l(3)95Ed4378 Normal embryos. Clones with rough eyes, bristle defects and wing nicks
l(3)95Ed4532 Clones with bristle defects and wing nicks.
l(3)95Ee2153 Abnormal embryos. Clones with bristle defects.
l(3)95Ee4454 Clones with rough eyes, wing nicks and vein and bristle defects
l(3)95EFa2707
l(3)95EFa1103 Normal embryos and clones
l(3)95EFb1807
l(3)95EFb2328 Slightly abnormal embryos.
l(3)95EFc1665 Normal embryos and clones
l(3)95EFc2201
l(3)95EFd1214 Slightly abnormal embryos
l(3)95EFd4182