Subject: Re: FlyBase Query
Dear Chihiro,
There seems to be some confusion in the source of the various UAS-gbb
lines. It is true that the I received three UAS-gbb lines from Mike
Hoffmann as noted below but these are NOT the lines that were used in
the Khalsa et al 1998 paper. In that paper, we described making a
construct and recovering four UAS-gbb transgenic lines. These lines
are distinct from the three made in the Hoffmann lab. Our construct
makes use of only gbb cDNA sequences, with the endogenous polyA
signal. We made the new lines because we were unable to rescue gbb
mutants with the fly stocks we received from Mike Hoffmann. It was
never clear why Mike's lines did not completely rescue gbb mutants in
our hands. I discussed the problem with Karen Staehling-Hampton at
one point but we never resolved the problem.
In the Khalsa etal 1998 paper, we unfortunately did not name the four
new lines we generated because for the experiments described there we
did not observe dramatic differences between the lines. However,
since that paper, when we have sent the lines out we have been
careful to denote the line number should it be important in future
experiments.
They were designated:
UASgbb9.1 on chr III
UASgbb9.9 on chr II
UASgbb9.7 on chr X
UASgbb9.8 on chr II
I hope this helps to clarify the confusion. Of course, if you have
any further questions please feel free to contact me.
Regards,
Kristi
\--
Kristi A. Wharton
Associate Professor
Department of Molecular Biology, Cell Biology and Biochemistry
Brown University
Box G-J160
Providence, R.I. 02912