Isolation and characterization of Df(2L)BSC112
Jennifer Deal and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC112 was isolated as a FLP recombinase-induced recombination event involving P{XP}d02040 and PBac{WH}f07749. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d02040/ PBac{WH}f07749 males crossed to w1118; P{w+mC=hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC112 from the segment of P{XP}d2040 to the left of its FRT site and the segment of PBac{WH}f07749 to the right of its FRT site. Exelixis, Inc. determined the insertion site of P{XP}d2040 to be at Release 3 genomic coordinate 18585060 on chromosome arm 2L and the insertion site of PBac{WH}f07749 to be at Release 3 genomic coordinate 18676894 on arm 2L. The Gene Disruption Project determined the insertion site of PBac{WH}f07749 to be at Release 3 genomic coordinate 18676916 on arm 2L. The cytological breakpoints of Df(2L)BSC112 predicted from these coordinates are 36F7;37A1. It failed to complement msl-1gamma216, Df(2L)Exel7071 and Df(2L)OD15 for lethality.